중국 및 일본산 후박 잎의 형태 및 성분 비교 = The Comparison of Morphological and Constituents of the leaves of Magnolia officinalis, M. biloba and M. obovata

다국어 초록 (Multilingual Abstract)

The stem bark and leaf of Magnolia have been used medical therapy of traditional medical system in China, Japan, and Korea for thousands of years.
Nowadays, Magnoliae Cortex used herbal medicine differ extremely significantly by region, leaf shape, DBH, tree height, crown size, age, bark thickness, color of bark powder, oiliness, grounding nature, bark type, position of sampling, etc., of which region, leaf shape, powder color, bark thickness and DBH are the most influential factors.
In this study, we analyzed and quantified the hexane fraction from the leaves of Magnolia species by HPLC. HPLC methods for the determination of the contents of magnolol (Ⅰ), honokiol (Ⅱ), obovatol (Ⅲ) of the hexane fraction from the leaves of Magnolia species were developed and validated by ICH guide lines comparing the linearity, intraday precision, inter-day precision. Those compounds were detected with optimal condition; wavelength: 288 nm, column: Zorbax Eclipse XDB-C18 (2.1 × 150mm, 5 μm), mobile phase: Acetonitril - 0.1% H3PO4 (45 : 55, v/v) buffer, flow rate: 0.5 ml/min.
The leaves of M. officinalis contained 1.08% Ⅰ, 2.37% Ⅱ, 0.57% Ⅲ and that of M. biloba contained 0.50% Ⅰ, 0.03% Ⅱ, 1.54% Ⅲ and that of M. obovata contained 0.37% Ⅰ, 0.03% Ⅱ, 0.76% Ⅲ.
The contents of effective ingredients were higher in the leaves of M. officinalis and M. biloba than M. obovata. Especially, the content of obovatol in the leaves of M. biloba was high remarkably. This show that the shapes of leave affect the contents of obovatol.

목차 (Table of Contents)

• Ⅰ. 서론
• Ⅱ. 재료 및 실험 방법
• 1. 생약재료
• 2. 시약 및 기기
• 3. 실험방법
• 3.1. 잎의 형태 비교
• 3.2. 표준품 추출 및 분리
• 3.3. 시료의 전처리 및 추출
• 3.4. 용매에 따른 추출 효율
• 3.5. 분석 방법
• 3.6. 분석법 검증
• 3.7. 후박 잎 중 유효성분의 분석
• Ⅲ. 결과 및 고찰
• 1. 잎의 형태 비교
• 2. 표준품 분리 및 동정
• 3. 분석법 검증
• 3.1. 유효성분 물질의 확인
• 3.2. 컬럼의 선정
• 3.3. 추출효율에 따른 분획의 선정
• 3.4. 직선성 및 범위
• 3.5. 정밀도
• 4. 중국 후박 및 일본 후박 잎으로부터의 유효성분 함량 분석
• Ⅳ. 결론
• Ⅴ. 참고 문헌
• Ⅵ. Abstract