Application of sperm selection using hyaluronic acid binding in intracytoplasmic sperm injection cycles : a sibling oocyte study

다국어 초록 (Multilingual Abstract)

Objective: To investigate whether sperm selection by hyaluronic acid (HA) binding could improve fertilization rate and embryo quality in ICSI(Intracytoplasmic sperm injection) cycles.

Methods: Two hundred nineteen oocytes obtained from eighteen women were injected with either HA-bound (n=107) or conventionally selected spermatozoa (n=112) in a randomized way. All of the participants were infertile couples who had normal sperm parameters but low fertilization rate in previous IVF cycle (n=5) or experienced multiple IVF failures (n=13).

Results: Lower fertilization (75.7% vs. 83.0%) and cleavage rate on day 2 (72.9% vs. 83.0%) was observed in oocytes injected with HA-bound spermatozoa than the conventional group, but the difference was not significant. Significantly lower cleavage rate was observed on day 3 in HA group (56.0% vs. 69.6%, P=0.038). Blastocyst formation rate and the number of transferred embryos were similar in both groups. In multiple IVF failure patients, significantly reduced fertilization rate (71.8% vs. 85.3%, P=0.046) and cleavage rate on day 2 (70.4% vs. 85.3%, P=0.029) and day 3 (53.5% vs. 77.3%, P=0.002) were noticed in HA group. Five women achieved pregnancy continuing more than 12 weeks after transfer (27.8%).

Conclusions: Success of ICSI was not related with the number of embryos fertilized by HA-bound spermatozoa. Application of ICSI by sperm selection using HA binding was not helpful in couples with repeated poor fertilization or implantation despite normal sperm parameters.

목차 (Table of Contents)

• Abstract 3
• Contents 5
• List of tables 6
• Introduction 7
• Material and Methods 9
• Results 13
• Discussion 18
• References 20
• Abstract in Korean 24