Analysis of quercetin glycosides from Nelumbo nucifera and phlorotannins from Ecklonia stolonifera by reversed-phase HPLC and LC-MS

목차 (Table of Contents)

• 1. INTRODUCTION = 1
• 1.1. Quercetins from Nelumbo nucifera = 1
• 1.2. Phlorotannins from Ecklonia stolonifera = 4
• 2. MATERIALS AND METHODS = 7
• 2.1. Analysis of the leaves of Nelumbo nucifera = 7
• 2.1.1. Materials and reagents = 7
• 2.1.2. Preparation of standard solutions = 9
• 2.1.3. Preparation of sample solutions = 9
• 2.1.4. HPLC analysis = 9
• 2.1.5. Validation of HPLC method = 10
• 2.1.6. LC-MS analysis = 10
• 2.2. Analysis of Ecklonia stolonifera = 10
• 2.2.1. Materials and reagents = 10
• 2.2.2. Preparation of standard solutions = 14
• 2.2.3. Prepartion of sample solutions = 14
• 2.2.4. HPLC analysis = 14
• 2.2.5. Validation of HPLC method = 15
• 2.2.6. LC-MS analysis = 15
• 3. RESULTS AND DISCUSSION = 16
• 3.1. Optimization of HPLC conditions of quercetin glycosides = 16
• 3.2. HPLC method validation of quercetin glycosides = 19
• 3.3. Identification of flavonoids in the extracts of N.nucifera = 25
• 3.4. Quantification of flavonoids in the extract of N.nucifera = 30
• 3.5. HPLC analysis and validation validation of Phlorotannins = 30
• 3.6. Identification of phlorotannins in the extracts of E. stolonifera = 37
• 3.7. Quantification of phlorotannins in the extracts of E.stolonifera = 42
• 4. Conclusions = 42
• 5. REFERENCES = 43
• 개요 = 46
• Abstract = 48