韓國 在來山羊의 反芻胃內 微生物의 分離·同定 및 Actinomyces sp.의 營養素 要求量에 관한 硏究 = Isolation & identification of rumen bacteria from Korean native goat and nutritional requirements of actinomyces sp.

다국어 초록 (Multilingual Abstract)

This study was undertaken to isolate the bacterial flora in the rumen of Korean native goat and to determine nutritional requirements of a new and the most predominant cellulolytic bacteria (Actinomyces) in the rumen.
The results which were obtained in this experiment are as follows:
1. The gram positive cocci which were isolated are Streptococcus bovis, Streptococcus faecium, Streptococcus intermedius, Sarcina sp., Ruminococcus albus and Ruminococcus flavefaciens.
The TATAC medium and Cellulose medium could be used successfully as a selective medium for the isolation of Streptococcus and Ruminococcus in the rumen, respectively.
2. The gram negative cocci which were isolated are Veillonella alcalescens, Megasphaera elsdenii and Quin's Oval.
The M-VS medium and PMS medium could be used successfully as a selective medium for the isolation of Veillonella and Megasphaera in the rumen, respective
3. The gram positive rods which were isolated are Lactobacillus brevis, Lactobacillus acidophilus, Bifidobacterium pseudolongum, Bifidobacterium thermophilum, Eubacterium aerofaciens, Eubacterium ruminantium and Actinomyces sp., The M-LBS medium, BS medium and CC-ES medium could be used successfully as a selective medium for the isolation fo Lactobacillus, Bifidobacterium and Eubacterium in the rumen, respectively.
4. The gram negative rods which were isolated are Bacteroides ruminicola subsp. ruminacola, Bacteroides ruminicola subsp. brevis, Bacteroides succinogenes, Fusobacterium necrophorum, Butyrivibrio fibrisolvens, Succinimonas amylolytica, Selenomonas ruminantium and Clostridium clostridiiforme.
The CC-NBGT medium and FS medium could be used successfully as a selective medium for the isolation of Bacteroides and Fusobacterium in the rumen, respectively.
5. Optimum pH and temperature for the growth of Actinomyces sp. 9RCC5 were 6.8-6.9 and 39℃, respectively.
6. Actinomyces sp. 9RCC5 was grown on the following nine substrates: fractose, galactose, glucose, glycogen, maltose, melibiose, raffinose, starch, sucrose. Starch was an excellent source of energy, and wide variations in substrate affinities were seen among the substrates which were utilized by this strain.
7. Casein hydrolysate was essential for the growth of Actinomyces sp. 9RRC5 ; this strain grew better with organic nitrogen source (ammonium sulfate) as the sole nnitrogen source. As well as casein hydrolysate, six amino acids (DL-valine, DL-leucine, glycine, DL-threonine, L-arginine, L-proline) could be used as nitrogen source for the growth of this strain.
8. Actinomyces sp. 9RCC5 was absolutely require riboflavin and pantothenate growth, and was stimulated by ρ-aminobenzoic acid and biotin for growth.
9. Actinomyces sp. 9RCC5 was required acetic acid as a essential growth factor and was stimulated by a combination of branched chain fatty acids (isobutyric acid, isovaleric acid, 2-methylbutyric acid).
10. Actinomyces sp. 9RCC5 was required Na^(+), K^(+), Ca^(2+) as essential growth factor (minerals) and PO□ Mg^(2+), Co^(2+) were needed for the growth of this strain.
11. Supplementation of clarified rumen fluid in the basal medium enhanced both the growth rate and the final cell yield of Actinomyces sp. 9RCC5.
12. Actinomyces sp. 9RCC5 was required hemin as an essential growth factor in this experimental condition.

목차 (Table of Contents)

• 目次 = ⅰ
• LIST OF TABLES = ⅳ
• LIST OF FIGURES = ⅵ
• LIST OF APPENDIX TABLES = ⅸ
• Ⅰ. 緖論 = 1
• Ⅱ. 硏究史 = 3
• 1. Rumen bacteria의 분리 및 동정 = 3
• 1) 혐기배양법 = 3
• 2) 분리배지 = 6
• 3) Rumen bacteria의 분리= 8
• 2. 섬유소분해균의 영양소 요구량 = 16
• 1) 에너지 = 16
• 2) 단백질 및 비단백태 질소화합물 = 16
• 3) 비타민 = 17
• 4) 휘발성지방산 = 18
• 5) 광물질 = 19
• 6) CO₂ = 20
• Ⅲ. 한국재래산양의 rumen bacteria의 分離 및 同定 = 21
• 1. 序論 = 21
• 2. 材料 및 方法 = 22
• 1) 공시동물 = 22
• 2) 시험사료 및 공시동물의 사양관리 = 22
• 3) 혐기배양장치 = 24
• 4) Rumen fluid 채취 및 시료제조 = 24
• 5) 사용배지 = 25
• 6) 혐기배양 = 26
• 7) 분리 및 동정 = 26
• 3. 結果 및 考察 = 31
• 1) Gram양성 구균의 분리 및 동정 = 31
• 2) Gram음성 구균의 분리 및 동정 = 37
• 3) Gram양성 간균의 분리 및 동정 = 39
• 4) Gram음성 간균의 분리 및 동정 = 44
• Ⅳ. Actinomyces sp. 9RCC5의 營養素要求量 = 52
• 1. 序論 = 52
• 2. 材料 및 方法 = 53
• 1) 배양조건 및 각 영양소 요구량 = 53
• 2) 에너지 공급원 = 53
• 3) 질소 공급원 = 55
• 4) 비타민 공급원 = 55
• 5) 휘발성지방산 공급원 = 56
• 6) 광물질 공급원 = 56
• 7) Rumen fluid 첨가 = 57
• 3. 結果 및 考察 = 57
• 1) 배양조건 및 각 영양소 요구량 = 57
• 2) 에너지원 요구량 = 59
• 3) 질소원 요구량 = 62
• 4) 비타민 요구량 = 68
• 5) 휘발성지방산 요구량 = 73
• 6) 광물질 요구량 = 78
• 7) Rumen fluid첨가 = 80
• Ⅴ. 要約 = 83
• LITERATURE CITED = 85
• APPENDIX = 105
• ABSTRACT = 128