미생물 관련 대사산물 및 생육조절인자의 작용기작

다국어 초록 (Multilingual Abstract)

A bactenal srain LC-413, producing an extracellular inulin fructotransferase (depolymerizing) which convers inulin into di-D-fructofuranose dianhydride (DFAIII, was isolated from soil Inulin fructotransferase from the isolate identified as a strain Fabobacterium sp was purified from the culture broth by ammonium sulfate precipitation, followed by column chronatograpies on DEAE-Toyopearl 650 M and phenyl-Toyopearl 650 M The purified enzyme gave a single band on an electrophoretic disc-gel The molecular weight of the enzyme was estimated to be 44,000 Da by SDS-polyacrylamide gel electrophoresis, and 45,000 Da by gel filtration, suggesting the monomeric state of the enzyme The isoelectric point of the enzyme was about pH 4 5 The optimal pH and temperature for the enzyme reaction were 60 and 50?, respectively The purified enzyme digested inulin into di-D-fructofuranose-1,2' 2,3'-di-anhydride, confirming the enzyme was an inulin fructotransferase (inulinase II)

목차 (Table of Contents)

• 1.Purification and Characterization of an Inulin Fructotransferase from Flavobacterium sp. LC-413
• 2. Tsukamurella sp. 26A에 의한 생물계면활성제의 생산
• 3. Lipase의 Transesterification반응에 의한 생물계면활성제의 합성
• 4. 유기용매계에서 Lipase에 의한 Fructose Ester의 합성
• 5.Purification and characterization of a novel levanoctaose-producing levanase from Pseudomonas sp. K-52
• 6. Lactobacillus sp. JC-7과 Lactobacillus acidophilus 88간의 세포융합주의 특성에 관한 연구