한국인의 CYP2D6(B)와 CYP2D6(T)의 유전적 다형성에 관한 연구 : (한국인 Debrisoquine 대사형의 분자역학적 연구) = PCR and RFLP-based CYP2D6(B) and CYP2D6(T) Genotyping for Korean Lung Cancer Cases and Controls

다국어 초록 (Multilingual Abstract)

The genetically determined CYP2D6 activity is considered to be associated with cancer susceptibility with inter-individual variation. Genetic polymorphism of CYP2D6(B) and CYP2D6(T) was determined by the two polymerase chain reaction(PCR) and BstN1 and EcoN1 restriction fragment length polymorphism(REFP) for 67 lung cancer cases and 95 healthy volunteer controls. The cases were composed of 26 squamous cell carcinoma, 14 small cell carconoma, 10 adenocarcinoma, 3 large cell undofferentiated carcinoma, and 14 not histologically diagnosed.
The result was gained from the 142 subjects (57 cases and 85 controls) who observed successfully in two PCR and BstN1/EcoN1 RFLP. Only one and no mutant allele of the CYP2D6(B) and CYP2D6(T) gene was detected, that is the frequency of mutant allele was very low; 0.7%(1/142) and 0%(0/142), respectively Detected mutant allele of the CYP2D6(B) was heterozygous type(WM). The odds ratios for lung cancer susceptibility with CYP2D6(B) and CYP2D6(T) genotype were not calculated This result was similar to the previous understanding that the mutant allele is very rare in Orientais compared to Caucasians, therefore, it considered that CYP2D6(B) and CYP2D6(T) genotype have maybe no association with lung cancer susceptibility in Koreans.
For the grneral characteristics, gender ratio(male/female) was 8.6(60/7) and 4.6(78/17), and the mean age was 58.8±9.6 years and 52.4±11.5 years in cases and controls(p<0.01), respectively. The prevalence of smoking and drinking was very high around 70-80% with no difference between the two groups(p>0.05), however, the amount(pack-years) of smoking was significantly heavier, and the duration of drinking was a little longer in cases than controls(p<0.001).

목차 (Table of Contents)

• Abstract
• 1.서론
• 2.연구방법
• 3.연구성적
• 4.고찰
• 5.결론
• 참고문헌