이산화티타늄 등 식품첨가물 미립자 평가 방법 개발 = Development of assessment tool to measure the engineered nanomaterials in food products

다국어 초록 (Multilingual Abstract)

Purpose
Development of a test method to separate and analyze food additive nanoparticles (SiO₂ and TiO₂)
- Literature review of food additive nanoparticles
- Survey of the commercial food additives (SiO2 and TiO2) and monitoring their size distribution.
- Development of pretreatment conditions for the analysis of food additive nanoparticles
- Development of test methods for analyzing food additive nanoparticles
Contents
1) Literature review of food additive nanoparticles
2) Survey of the commercial food additives (SiO2 and TiO2) and monitoring their size distribution.
3) Development of qualitative and quantitative analytical methods to investigate food additive nanoparticles
4) Establishment of pretreatment conditions using simulated food matrices
5) Separation of food additive nanoparticles using magnetic separation technology
6) Seperation of food additive nanoparticles using Field-Flow Fractionation (FFF)
7) Expert meeting for the developed test methods
8) Size fractionation of food additive nanoparticles using sucrose density gradient sedimentation.
9) Validation of the developed method using commercial food products
Results
1) Publications of the results in SCI journal
- ‘Separation and characterization of food additive nanoparticles (TiO2 and SiO2) in commercial food products’ (expected until 2019.08).
2) Presentation of the results in scientific symposium
- ‘Size distribution and cytotoxicity of food additive titanium dioxide and silicon dioxide’
- ‘Development of sucrose density gradient sedimentation to separate different size of TiO2 and SiO2 nanoparticles in food’
- ‘Development of assessment tool to measure the nano-sized titanium dioxides in food products by magnetic separation’
- ‘Establishment of a method to quantify TiO2 and SiO2 nanoparticles in food’
3) Assay development
- Monitoring nanoparticles in food additive
- Monitoring food additive nanoparticles in commercial food products
Expected Contribution
1) The methods developed in this study for the separation and detection of nanoparticles in food product could be used for monitoring the nanoparticles in various food products, which would provide essential means to control and regulate the safe use of nanoparticles in food products.
2) The sample preparation methods will serve as a standard method to retrieve nanoparticles from complicated food matrix.

국문 초록 (Abstract)

본 연구팀은 대표적인 식품첨가물로서 이산화티타늄과 이산화규소를 선정하였으며, 이들에 대한 제외국 관리현황과 관련 문헌 자료를 수집함. 수집된 자료를 토대로 식품으로부터 식품첨가물 나노입자를 검출하기 위한 시험법을 정립함. 또한 자성분리 기술을 접목하여, 식품의 복잡한 매트릭스를 극복하여 나노입자를 분리 검출하는 신기술을 개발함. 정립된 기존의 검출법과의 비교분석을 통해, 새롭게 제안된 자성분리 기술을 이용하여 복잡한 성분으로 구성된 실제 식품으로부터 효과적으로 식품첨가물을 분리 검출하는데 성공하였음. 본 과제를 통해 도출된 검출 기술을 바탕으로, 실제 식품 첨가물 나노입자 검출을 위한 표준 시험법을 확립하였음. 본 과제를 통해 개발된 식품첨가물의 시험법은 향후 식품에 첨가된 식품첨가물의 나노물질 안전 관리 및 규제안 마련에 활용될 것으로 판단됨.