쥐 척수후근 신경절 세포의 칼슘 전류 : (Perforated Patch 기법을 이용한 연구) (a study by the perforated patch technique) = Calcium-current in rat dorsal ganglion neuron

다국어 초록 (Multilingual Abstract)

Perforated patch clamp technique was used to record and characterize the Ca currents in rat dorsal root ganglion neuron. Primary culture was performed from the rat at postnatal weeks 4-5.
The following results were obtained.
1. Single ganglionic cells were distinguished from their sizes into small, medium and large. In these experiments only the medium-sized cells were used.
2. Various low-threshold and/or high-threshold Ca currents were recorded and the low-threshold currents appeared more complex pattern.
3. In having low-threshold Ca current cells the most common pattern was about 30-50% amplitudes of high-threshold Ca current.
4. In the case of low-threshold Ca current the permeability of Ba ion was lower than high-threshold one.
5. When the membrane potential was holded at -40mV, the depolarizing pulse might induce Ca currents with rundown tendency time-dependently.
6. Various Ca channel blockers were used to confirm their potencies and characteristics. Although some blocking effects were observed, the specific effects of each blockers were seldom found.
7. Applications of 8-bromo-cGMP and CPT-cGMP decreased the Ca currents and the effects of 8-bromo-cGMP were more reversible.

목차 (Table of Contents)

• Abstract
• 서론
• 실험재료 및 방법
• 결과
• 고찰
• 결론
• 참고문헌