식물로부터 Thaumatin-like protein 유전자의 클로닝과 분비과정 및 유용농작물에서의 발현에 의한 내병성 작물개발

다국어 초록 (Multilingual Abstract)

Two new members(Bsarla and Bsarlb) of the Sarl gene family have been identified from a flower bud cDNA library of Brassica campestris and their functional characteristics were amalyzed. The two clones differ from each other at 14 positions of the 193 amino acid residues deduced from their coding region. The amino acid sequences of Bsarla and Bsarlb are most closely related to the Sarl family, genes that function early in the process of vesicle budding from the endoplasmic reticulum(ER). The sequences contanin all the conserved motifs of the Ras superfamily (G1-G4 motifs) as well as the distinctive structural near the C-terminus that is Sarl specific. Our phylogenetic analysis confirmed that these two clones can indeed be considered members of the Sarl family and that they have a close relationship to the ARF family. The Bsarl proteins, expressed in Escherichia coli, cross-reacted with a polyclonal antibldy prepared against Saccharomyces cerevisiae Sar I protein, It also exhibited GTP-binoing activity Genomic Southern blot analysis, using the 3'-gene-specific regions of the Bsarl cDNAs as probes, revealed that the two cDNA clones are members of a B. campestris Sarl family that consists of 2 to 3 genes. RNA blot analysis, using the same gene-specific probes, showed that both genes are expressed with similar patterns in most tissues of the plant, including leaf, stem, root, and flower buds. Furthermore, when we placed the two Bsarl genes under the control of the yeast pGKI promoter into the temperature-sensitive mutant yeast strain S. cerevisiae Sec 12-1, they suppressed the mutation which consists of a defect in vesicle transport. The amino acid sequence similarity, the GTP-binding activity, and the functional suppression of the yeast mutation suggest that the Bsarl proteins are functional homologues of the Sarl protein in S. cerevisiae and that they may perform similar biological functions.

목차 (Table of Contents)

• Purification and Characterization of an Antifungal PR-5 Protein from Pumpkin Leaves
• References
• The presence of a Sarl gene family in Brassica campestris that suppresses a yeast vesicular transport mutation Sec12-1
• Abstract
• 1.Introduction
• 2.Discussion
• Purification of an antifungal PR-5 protein flower buds of Brassica campestris and cloning of its gens
• Abstract