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For the developmemt of B subtilis host and sector system for the overexpression of substifism and the therapeutic protein such as staphylokinase (SAK) sigma factor mutants were constructed Among the spore mutants(spoIIA, spoIIIC, spoIIG and its double...
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subtilisin 및 staphylokinase 과발현을 위한 고초균 숙주 및 벡터계의 개발
한글로보기부가정보
다국어 초록 (Multilingual Abstract)
For the developmemt of B subtilis host and sector system for the overexpression of substifism and the therapeutic protein such as staphylokinase (SAK) sigma factor mutants were constructed Among the spore mutants(spoIIA, spoIIIC, spoIIG and its double mutation), spoIIG single mutants shows the highest activity in the subtilism production Single copu lacZ fusion sectors with different promoter (P43 known as vegetative promoter, and aprE, amyE known as stationary phase promoters) were compared to screen the best expression system Expression level of p43-tacZ and amyE-lacZ were higher than that of aprE-lacZ P43 promoter were selected for the SAK production in the next year.
For the developmemt of B subtilis host and sector system for the overexpression of substifism and the therapeutic protein such as staphylokinase (SAK) sigma factor mutants were constructed Among the spore mutants(spoIIA, spoIIIC, spoIIG and its double mutation), spoIIG single mutants shows the highest activity in the subtilism production Single copu lacZ fusion sectors with different promoter (P43 known as vegetative promoter, and aprE, amyE known as stationary phase promoters) were compared to screen the best expression system Expression level of p43-tacZ and amyE-lacZ were higher than that of aprE-lacZ P43 promoter were selected for the SAK production in the next year.
목차 (Table of Contents)
- Subtilisin 및 Staphylokinase과 발현을 위한 고초균 숙주 및 벡터계의 개발
- 1.서 론
- 2.실 험
- 3.결과 및 토의
- 4.참 고 문 헌
- Subtilisin 및 Staphylokinase과 발현을 위한 고초균 숙주 및 벡터계의 개발
- 1.서 론
- 2.실 험
- 3.결과 및 토의
- 4.참 고 문 헌
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