Expression and functional characterization of recombinant chicken interferon-gamma = 닭 인터페론 감마의 발현 및 기능에 관한 연구

다국어 초록 (Multilingual Abstract)

A cDNA encoding chicken interferon-gamma(chIFN-??) was cloned from a CD4+ T-cell hybndoma gy reverse transcription-polymerase chain rcaction (RT-PCR) and expressed in Escherichia coli, COS- and CEC-32 filbroblast cell lines. In general, recombinant chicken IFN-??(rchIFN-??) expressed in the COS- and CEC-32 cell lines showed high bioactivity in vitro. The kinetics of IFN-?? gene expression were cxamined in concanavalin A(Con A)-activated spleen lymphocytes hy Noithern bolt and RT-PCR. IFN-?? mRNA was detecred as early as 30 min after Con A activation, reached peak expression at 2 h and then deereased starting at 4 h post Con A activation. A rabbit serum made to a syntbetic peptide of IFN-??(MBP-IFN) and a 26-27 kDa secreted protein from COS cells and Con A-activated spleen cells. IFN-?? inhibited vesicular stomatitis virus(VSV) mediated cytotoxicity of chicken embryonic fibroblast(CEF) cells and upregulated the expression of many macrophage cell surface antigens, including class I and class II major hitocompatibility complex (MHC) proteins. These resulcs show that chicken IFN-?? possesses anti-viral activity and imniunoregulates macrophage activities. ⓒ 1997 Elsevier Science B. V.

목차 (Table of Contents)

• Abstract
• 1.Introduction
• 2.Materials and methods
• 3.Results
• 4.Discussion
• References