Aspergillus nidulans 의 tRNA 유전자의 구조와 발현에 관한 연구 ( Ⅶ ) Aspergillus nidulans의 initiator methionyl - tRNA 유전자의 분자구조 = Studies on the organization and Expression of tRNA Gene ( Ⅶ ) The Structure of the Initiator methionyl - tRNA Gene of Aspergillus nidulans

다국어 초록 (Multilingual Abstract)

A specific tRNA gene clone (named pANt86) was isolated by colony hybridization to the total tRNA gene bank with a certain tRNA probe extracted from Aspergillus nidulans. The 485 nucleotide long sequence containing this gene was determined by the Maxam and Gilbert sequencing method. The sequence analysis reveals that this tRNA is methionyl-tRNA(CAU). Compared with other initiator tRNA^(Met) gene for the sequence homology and two-positional discriminators, this tRNA gene is initiator tRNA^(Met). The nucleotide sequences of this tRNA gene was compared with those of other organisms. From this, the intragenic control regions constituted by two separate transcriptional signals(Box A and Box B) are located in the regions of highly conserved sequences. And this gene reveals several conserved upstream sequences, the ACT-TA box, TA(C)TAT, AATTTT, and TTC. The T clusters are also found in the 3`-flanking region. And an evolutionary distance matrix was obtained and a phylogenetic tree based on this matrix was constructed. This phylogenetic tree reveals that extant organisms are found to fall into three phylogenetically coherent groups, the eukaryotes, the eubacteria, and the archaebacteria.