젖산균 발효 천문동의 항균활성 및 발효 특성 = Antimicrobial Activity and Characteristics of Asparagus Cochinchinensis Fermented With Lactic Acid Bacteria

국문 초록 (Abstract)

호흡기 질환 개선에 도움을 주는 천문동의 젖산균 발효 및 호흡기 병원성 미생물에 대한 항균활성 특성을 확인하고자 연구를 진행하였다. 발효 전 천문동과 젖산균 발효천문동의 항균활성은 추출물 별로 차이가 나타났고 EtOAc추출물에서 항균활성은 더욱 개선된 것을 확인할 수 있었다. EtOAc 추출물에서 MIC는 생육 저해환이 발효 전 천문동의 MIC가 E. coli는 100 ㎎/mL, S. epidermidis는 75 ㎎/mL, S. aureus에서는 100 ㎎/mL의 농도였던 것이 ACLM은 E. coli에서 25 ㎎/mL, ACLS는 S.epidermidis에서 12.5㎎/mL, ACLP는 S.aureus에서 12.5 ㎎/mL로 나타났다. 이러한 결과로부터 젖산균 발효를 통하여 천문동의 항균활성이 크게 증가하고 호흡기 질환에 효과가 있을 것이라 기대할 수 있다. ACLM와 ACLS의 경우 생균수, pH, 산도가 비슷한 경향을 나타낸 반면 ACLP은 120시간 발효 시에도 생균의 수는 감소가 크지 않고 거의 일정하게 유지되고 pH, 산도는 각각 48시간까지 일정하게 감소 또는 증가하다 일정해지는 형태를 나타내었다. 일반성분 중 수분과 회분은 천문동 발효 전·후에서 큰 차이가 없었다. 총당과 환원당은 발효 전 천문동에 비하여 발효 후 그 값이 크게 감소하여 미생물의 발효에 당이 사용되었음을 추측할 수 있었다.

다국어 초록 (Multilingual Abstract)

Asparagus cochinchinensis is known to ameliorate respiratory disease. We evaluated the antimicrobial activity of non-fermented and fermented A. cochinchinensis using lactic acid bacteria and investigated the physicochemical characteristics of the fermented A.cochinchinensis. Non-fermented A.cochinchinensis showed lower levels of antimi-crobial activity than the fermented A.cochinchinensis. Ethyl acetate (EtOAc) extracts of the fermented A. cochinchin-ensis showed antimicrobial activities against the indicator strains at lower minimum inhibitory concentration (MIC). The MIC of the EtOAc extract of non-fermented A. cochinchinensis against Escherichia coli, Staphylococcus epi-dermidis, and Stphylococcus aureus were 100, 75, and 100 ㎎/ml. In contrast, the MIC of the EtOAc extract of ACLM was 25 ㎎/mL against E. coli. The MIC of the EtOAc extract of ACLS was 12.5 ㎎/mL against S.epi-dermidis, and the MIC of the EtOAc extract ofACLP was 12.5 ㎎/mL against S.aureus. The viable cell number, pH, and acidity of A. cochinchinensis fermented by Lactobacillussakei 383 were similar to those of A. cochinchin-ensis fermented by Leuconostocmesenteroides 4395, but were different from those of A. cochinchinensis fermented by Lactobacillus.plantarum KCCM 11322. These results suggested that the fermented A. cochinchinensis by lactic acid bacteria may be a good candidate for application to ameliorate respiratory disease.

참고문헌 (Reference)

1 양지영, "허브추출물의 항균 작용" 한국식품영양과학회 34 (34): 1130-1135, 2005

2 정민기, "천문동 추출물의 호흡기 세균에 대한 항균활성 및 항산화" 한국미생물·생명공학회 43 (43): 367-372, 2015

3 이승연, "천문동 추출물에 의한 조골세포 분화 촉진 및 파골세포 생성 억제효과" 한국식품영양과학회 37 (37): 16-19, 2008

4 강동희, "유산발효에 의한 발효한약의 기능분석" 한국미생물·생명공학회 39 (39): 259-265, 2011

5 서주희, "서울지역의 미세먼지가 호흡기계 질환으로 인한병원입원에 미치는 영향" 한국대기환경학회 22 (22): 564-573, 2006

6 최수빈, "상산나무 잎 추출물의 항균활성 및 안정성 조사" 한국식품과학회 46 (46): 39-43, 2014

7 장명숙, "매실즙이 열무 물김치의 발효숙성에 미치는 영향" 한국식품조리과학회 20 (20): 511-519, 2004

8 김로의, "두유 커드를 생산하는 김치 유래 젖산균의 동정" 한국생명과학회 21 (21): 235-341, 2011

9 Dunnette SL, "Washington JA. Microbiologic analysis of nasal polyp tissue" 78 : 102-108, 1986

10 Miller GL, "Use of dinitrosalicylic acid reagent for determination of reducing sugar" 31 : 426-428, 1959

11 Jung KH, "The effects of the standardized herbal formula PM014on pulmonary inflammation and airway responsiveness in a murine model of cockroach allergen-induced asthma" 155 : 113-122, 2014

12 Olsen A, "The antimicrobial activity of lactic acid bacteria from fermented maize (kenkey) and their interactions during fermentation" 79 : 506-512, 1995

13 정현건, "The New Phytoformula Containing Morus alba, Schizandra sinensis and Asparagus cochinchinensis Inhibits Lung Inflammation in vitro and in vivo" 한국생약학회 22 (22): 70-75, 2016

14 Du XH, "Studies on the isolation of antitumor active constituents of polysaccharides from Chinese herb Asparagus cochinchinensis (Lour.) Merr. [J]" 7 : 197-201, 1990

15 Tenji K, "Studies on the constituents of Asparagi Radix. On the structures of furostanololigosides of Asparagus cochinchinensis (Loureio) Merrill [J]" 27 : 3086-3094, 1979

16 Zhu GL, "Steroidal saponins from the roots of Asparagus cochinchinensis" 12 : 213-217, 2014

17 Bachert C, "Staphylococcus aureus superantigens and airway disease" 2 : 252-258, 2002

18 Davis MF, "Staphylococcus aureus colonization is associated with wheeze and asthma among US children and young adults" 135 : 811-, 2015

19 Park G, "Report of air quality in Busan 2012" Busan Metropolitan City Institute of Health & Environment 142-159, 2012

20 Kang SK, "Relationship of air pollution and pediatric respiratory diseases in Incheon city" 11 : 354-362, 2001

21 Son HL, "Phytochemical composition, in vitro antioxidant and anticancer activities of quercetin from methanol extract of Asparagus cochinchinensis (LOUR.) Merr" 7 : 3360-3366, 2013

22 AOAC, "Official methods of analysis"

23 Lee JH, "Methyl protodioscin from the roots of Asparagus cochinchinensis attenuates airway inflammation by inhibiting cytokine production" 2015 : 2015

24 Karovičová J, "Lactic acid fermented vegetable juices" 30 : 152-158, 2003

25 Perdigón G, "Lactic acid bacteria and their effect on the immune system" 2 : 27-42, 2001

26 Rafter J, "Lactic acid bacteria and cancer: mechanistic perspective" 88 : S89-S94, 2002

27 Koo HN, "Inhibition of tumor necrosis factor-α-induced apoptosis by Asparagus cochinchinensis in Hep G2 cells" 73 : 137-143, 2000

28 Ha EH, "Infant susceptibility of mortality to air pollution in Seoul, South Korea" 111 : 284-290, 2003

29 Irshad S, "In vitro antibacterial activities of three medicinal plants using agar well diffusion method" 2 : 1-8, 2012

30 Zaghloul AA, "Honey, a prospective antibiotic: extraction, formulation, and stability" 56 : 643-647, 2001

31 Igusa R, "Escherichia coli pneumonia in combination with fungal sinusitis and meningitis in a tsunami survivor after the Great East Japan Earthquake" 227 : 179-184, 2012

32 Lee HJ, "Dioscin and methylprotodioscin isolated from the root of Asparagus cochinchinensis suppressed the gene expression and production of airway MUC5AC mucin induced by phorbol ester and growth factor" 22 : 568-572, 2015

33 Dubois M, "Colorimetric method for determination of sugar and related substance" 28 : 350-352, 1956

34 Valderrey AD, "Chronic colonization by Pseudomonas aeruginosa of patients with obstructive lung diseases: cystic fibrosis, bronchiectasis, and chronic obstructive pulmonarydisease" 68 : 20-27, 2010

35 Hong KP, "Changes in microorganisms, enzymes and texture of Dongchimi by high hydrostatic pressure treatment" 30 : 596-601, 1998

36 Zhang HJ, "Bioactive Constituents from Asparagus cochinchinensis" 67 : 194-200, 2004

37 Shin SY, "Antibacterial activity of bioconvert edeicosapentaenoic (EPA) and docosahexaenoic acid (DHA) against foodborne pathogenic bacteria" 113 : 233-236, 2007

38 Cross ML, "Anti-allergy properties of fermented foods: an important immunoregulatory mechanism of lactic acid bacteria?" 1 : 891-901, 2001

39 Yang Shen, "A New Furostanol Saponin from Asparagus cochinchinensis" 대한약학회 34 (34): 1587-1591, 2011