목 적: Small interfering RNA (siRNA)를 이용하여 homeobox (HOXA) 10 유전자의 발현이 억제된 일차배양 자궁내막 세포를 이용하여 자궁내막 탈락막화 (decidualization)에 HOXA유전자를 포함한 세포 내 신호전...
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https://www.riss.kr/link?id=A100815910
이창세 (관동대학교 의과대학 제일병원 분자종양학 연구실) ; 박동욱 (관동대학교 의과대학 제일병원 생식생물학 및 불임연구실) ; 박찬우 (관동대학교) ; 김태진 (관동대학교 의과대학 제일병원 산부인과) ; Lee, Chang-Se ; Park, Dong-Wook ; Park, Chan-Woo ; Kim, Tae-Jin
2010
Korean
KCI등재,SCOPUS,ESCI
학술저널
207-216(10쪽)
0
0
상세조회0
다운로드국문 초록 (Abstract)
목 적: Small interfering RNA (siRNA)를 이용하여 homeobox (HOXA) 10 유전자의 발현이 억제된 일차배양 자궁내막 세포를 이용하여 자궁내막 탈락막화 (decidualization)에 HOXA유전자를 포함한 세포 내 신호전...
목 적: Small interfering RNA (siRNA)를 이용하여 homeobox (HOXA) 10 유전자의 발현이 억제된 일차배양 자궁내막 세포를 이용하여 자궁내막 탈락막화 (decidualization)에 HOXA유전자를 포함한 세포 내 신호전달기전을 분석하고자 하였다. 연구방법: 본원 산부인과에서 자궁내막 질환 이외의 이유로 전자궁 적출술을 받은 환자의 자궁내막 조직을 채취한다. $37^{\circ}C$에서 20분간 Trypsin-EDTA를 처리하여 단일세포로 분리한 후 10% fetal bovine serum이 첨가된 DMEM/F12 배지를 이용하여 24시간 동안 $37^{\circ}C$ 5% $CO_2$ 배양기 안에서 배양한다. 배양된 자궁내막 세포를 HOXA10 siRNA로 첨가한 후 TGF-${\beta}1$을 10 ng/mL 농도로 48시간 첨가하여 탈락막화를 유도한다. 배양된 자궁내막 세포에서 reverse transcription polymerase chain reaction을 이용하여 HOXA10, prolactin, cyclooxygenase (COX)-2, peroxisome proliferator-activated receptor (PPAR)-$\gamma$ 및 wingless-type MMTV integration site family (Wnt)의 발현을 관찰하였다. 결 과: HOXA10의 경우 transforming growth factor (TGF)-${\bata}1$과 HOXA10 siRNA를 처리하지 않은 대조군에 비하여 TGF-${\beta}1$을 처리한 군에서 약 1.8배 가량 발현양의 증가를 보였다. 자궁내막 탈락막 표지인자로 알려져 있는 prolactin의 경우 TGF-${\beta}1$을 처리한 경우 대조군에 비하여 유의한 발현의 증가를 보였으며 HOXA10 siRNA를 처리한 군에 있어서는 TGF-${\beta}1$을 첨가하더라도 prolactin mRNA의 발현양의 증가를 관찰할 수 없었다. 또한 자궁내막 세포의 분화인자로 알려져 있는 COX-2의 발현 역시 HOXA10 siRNA를 처리한 군에 있어서 mRNA 발현양이 유의하게 감소하였으며 TGF-${\beta}1$을 처리하여도 발현의 증가를 관찰할 수 없었다. Wnt4의 경우 HOXA10 siRNA를 이용하여 HOXA10의 발현을 억제한 경우 대조군에 비하여 유의하게 mRNA의 발현양이 감소하였으며 이러한 발현양의 감소는 TGF-${\beta}1$을 처리하여도 증가됨을 관찰할 수 없었다. PPAR$\gamma$의 발현은 HOXA10 siRNA의 처리와 관계없이 TGF-${\beta}1$에 의하여 감소하는 것을 관찰할 수 있었다. 결 론: Progesterone에 의하여 자궁내막 상피세포에서 분비되는 것으로 알려져 있는 TGF-${\beta}1$에 의한 자궁내막 기질세포의 분화 (탈락막화)는 HOXA10 및 Wnt에 의하여 조절되는 것으로 생각된다.
다국어 초록 (Multilingual Abstract)
Objective: This study was performed to clarify the role of HomeoboxA (HOXA) and its related signaling molecules in the decidualization of primary cultured endometrial cells. Methods: Human endometrial tissues were obtained by curettage of hysterectomy...
Objective: This study was performed to clarify the role of HomeoboxA (HOXA) and its related signaling molecules in the decidualization of primary cultured endometrial cells. Methods: Human endometrial tissues were obtained by curettage of hysterectomy specimens from patients with conditions other than endometrial diseases. Tissues were minced and digested with Trypsin-EDTA for 20 min, $37^{\circ}C$. Cells were cultured with DMEM/F12 medium in $37^{\circ}C$, 5% $CO_2$ incubator for 24 hrs. Cells were treated with HOXA10 siRNA and added transforming growth factor (TGF)-${\beta}1$ (10 ng/mL) for 48 hrs to induces decidualization in vitro. Reverse transcription polymerase chain reaction analysis was accomplished to observe the expression of HOXA10, prolactin, cyclooxygenase (COX)-2, peroxisome proliferatoractivated receptor (PPAR)-$\gamma$, and wingless-type MMTV integration site family (Wnt). Results: HOXA10 expression was increased (1.8 fold vs. non-treated control) in TGF-${\beta}1$ treated cells. Decidualization marker, prolactin, was significantly increased in TGF-${\beta}1$ treated cells compared with HOXA10 siRNA treated cells. Endometrial cell differentiation marker, COX-2 was down-regulated by HOXA10 siRNA even if cells were treated with TGF-${\beta}1$. Wnt4 was down-regulated by treated with HOXA10 siRNA, this expression patters was not changed by TGF-${\beta}1$. Expression of PPAR-$\gamma$ was down regulated by TGF-${\beta}1$ in regardless of HOXA10 siRNA treatment. Conclusion: TGF-${\beta}1$ which is induced by progesterone in endometrial epithelial cells may induces stromal cell decidualization via HOXA10 and Wnt signaling cascade.
참고문헌 (Reference)
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9 Kim MR, "Progesterone-dependent release of transforming growth factor-beta1 from epithelial cells enhances the endometrial decidualization by turning on the Smad signalling in stromal cells" 11 : 801-808, 2005
10 Quinn CE, "Pinopodes are present in Lif null and Hoxa10 null mice" 88 : 1021-1028, 2007
1 Hayashi K, "Wnt genes in the mouse uterus: potential regulation of implantation" 80 : 989-1000, 2009
2 Han C, "Transforming growth factor-beta (TGF-beta) activates cytosolic phospholipase A2alpha (cPLA2alpha)-mediated prostaglandin E2 (PGE)2/ EP1 and peroxisome proliferator-activated receptor-gamma (PPAR-gamma)/Smad signaling pathways in human liver cancer cells. A novel mechanism for subversion of TGF¬beta-induced mitoinhibition" 279 : 44344-44354, 2004
3 Chang HJ, "Transforming growth factor (TGF)-beta1-induced human endometrial stromal cell decidualization through extracellular signal-regulated kinase and Smad activation in vitro: peroxisome proliferator-activated receptor gamma acts as a negative regulator of TGF-beta1" 90 : 1357-1365, 2008
4 Taylor HS, "The role of HOX genes in human implantation" 6 : 75-79, 2000
5 Polakis P, "The many ways of Wnt in cancer" 17 : 45-51, 2007
6 Tamaya T, "The biologic role of sex steroid receptors in the decidualization of human endometrium" 11 : 573-578, 1985
7 Guo X, "Signaling cross-talk between TGF-beta/ BMP and other pathways" 19 : 71-88, 2009
8 Satokata I, "Sexually dimorphic sterility phenotypes in Hoxa10-deficient mice" 374 : 460-463, 1995
9 Kim MR, "Progesterone-dependent release of transforming growth factor-beta1 from epithelial cells enhances the endometrial decidualization by turning on the Smad signalling in stromal cells" 11 : 801-808, 2005
10 Quinn CE, "Pinopodes are present in Lif null and Hoxa10 null mice" 88 : 1021-1028, 2007
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20 Takekida H, "Autoradiographic studies on the nucleic acid and protein synthesis and the decidualization of endometrium in early pregnancy of rat" 18 : 200-213, 1971
21 Pinsonneault J, "A model for extradenticle function as a switch that changes HOX proteins from repressors to activators" 16 : 2032-2042, 1997
22 Taylor HS, "A conserved Hox axis in the mouse and human female reproductive system: late establishment and persistent adult expression of the Hoxa cluster genes" 57 : 1338-1345, 1997
Comparison between GnRH Antagonist and Agonist Long Protocols in Poor Responders
클로미펜을 이용한 배란 유도 후 임신된 다낭성 난소 증후군 여성에서 발견된 병합임신: 양측 난관임신 및 쌍태아 임신 1예
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2024 | 평가예정 | 해외DB학술지평가 신청대상 (해외등재 학술지 평가) | |
2021-01-01 | 평가 | 등재학술지 선정 (해외등재 학술지 평가) | |
2020-12-01 | 평가 | 등재후보로 하락 (해외등재 학술지 평가) | |
2012-01-05 | 학술지명변경 | 한글명 : 대한생식의학회지 -> Clinical and Experimental Reproductive Medicine외국어명 : The Korean Journal of Reproductive Medicine -> Clinical and Experimental Reproductive Medicine | |
2010-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2007-08-28 | 학술지명변경 | 한글명 : 대한불임학회지 -> 대한생식의학회지외국어명 : The Korean Journal of Fertility and Sterility -> The Korean Journal of Reproductive Medicine | |
2007-08-28 | 학회명변경 | 한글명 : 대한불임학회 -> 대한생식의학회영문명 : Korean Society For Fertility And Sterility -> The Korean Society for Reproductive Medicine | |
2007-01-01 | 평가 | 등재학술지 선정 (등재후보2차) | |
2006-01-01 | 평가 | 등재후보 1차 PASS (등재후보1차) | |
2004-07-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0 | 0 | 0 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0 | 0 | 0 | 0 |