쥐 해마의 Lipopolysaccharide 국소주입에 의한 Tumor Necrosis Factor-α, Interleukin-1β 및 Inducible Nitric Oxide Synthase의 발현 = Expression of Tumor Necrosis Factor-α, Interleukin-1β and Inducible Nitric Oxide Synthase after Stereotaxic Injection of Lipopolysaccharide in Rat Hippocampus

다국어 초록 (Multilingual Abstract)

Background : Brain inducible nitric oxide synthase (iNOS) might be detectable in several
pathologic conditions, and it is thought to play an important role in their pathophysiology.
Tumor necrosis factor (TNF)- and interleukin (IL)-1 are believed to be essential factors of
iNOS induction of the brain. Methods : After intrahippocampal stereotaxic injection of lipopolysaccharide
(LPS), the rat brains were removed at 6, 12 and 24 h. The rat brain tissues were
examined to clarify the expression patterns of TNF- , IL-1 and iNOS. Results : The inflammatory
cells which were stained with anti-TNF- antibody, appeared in 6 h and increased
for 24 h after LPS injection. The iNOS positive cells appeared after 12 h of LPS injection. A
semiquantitative analysis of reverse transcription-polymerase chain reaction (RT-PCR)
revealed that the TNF- and IL-1 mRNA arose at 1 h, peaked at 6 h and then declined
until 48 h after LPS injection. The iNOS mRNA arose after 6 h, peaked at 12 h, and declined
until 48 h after LPS injection. Conclusions : We conclude that the induction of inflammatory
events by intrahippocampal injection of LPS activates TNF- and IL-1 secretion, and this
is followed by an induction of iNOS expression. TNF- and IL-1 seem to be related with
iNOS expression in brain inflammation.

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