In vivo evaluation on the safety of DMZ-109, 121 and 129 in Balb/c mice

다국어 초록 (Multilingual Abstract)

To evaluate the safety of DMZ-109, 121 and 129, natural herb effective in anti-allergic, we performed the single oral dose toxicity test in Balb/c mice. In order to calculated 50% lethal dose (LD50) and approximate lethal dose (LD), test articles were once orally administered to male and female Balb/c mice. The animals were distributed into 8 groups of 5 mice per group according to their body weight. After administration with each sample (500, 1000 and 2000 mg/kg), the mortality and changes on body weight, clinical signs, gross observation, organ weights (heart, lung, liver, spleen, kidney and reproductive organs) were monitored 14 days after administration of DMZ-109, 121 and 129. We also conducted hematolocial test and serum clinical chemistry test (glucose, TP, ALB, AST, ALT, T-bil, total cholesterol, TG, LDL, HDL, LDH, D-bil and UA) after animal sacrifice. Any mortalities, test article related clinical signs and gross findings, significant body and organ weight changes, hematolocial changes and serum chemistry changes were not found. The results obtained in this study suggest that LD 50 and approximate LD in mice after single oral dose of DMZ-109, 121 and 129 were speculated over 2000 mg/kg in both male and female mice.