Antioxidant Activity of the Chemical Constituents from the Flower Buds of Magnolia denudata

다국어 초록 (Multilingual Abstract)

The scavenging activity of the flower buds of Magnolia denudata Desrousseaux on reactive oxygen species (ROS) was evaluated using 2',7'-dichlorofluorescin diacetate (DCFH-DA) in HT 1080 cells. Methanol (MeOH)and dichloromethane (CH2Cl2) extracts inhibited dosedependently generation of ROS in the cellular system.
MeOH and CH2Cl2 extracts were combined and fractionated with n-hexane, 85% aqueous MeOH, and n-butanol (n-BuOH). Both n-hexane-soluble and 85% aqueous-soluble fractions showing strong radical-scavenging activity in the cellular system were further separated by diverse chromatographic methods to give five known lignans (1-5). All these compounds exhibited significant radical-scavenging effect on intracellular ROS in a dose-dependent manner.
Their scavenging activity on various reactive oxygen species (ROS) was also evaluated using electron spin resonance (ESR) spin-trap techniques.

참고문헌 (Reference)

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