키토올리고당 함유 해양심층수에 의한 지방산 합성, 산화 조절 및 AMPK 활성 증가효과 = Effect of Deep Sea Water containing Chitosan Oligosaccharides on Fatty Acids Synthesis, β-oxidation, and AMPK Activation

국문 초록 (Abstract)

본 연구에서는 키토올리고당 함유 해양심층수가 지방세포의 지방산 합성을 억제하고 지방산 산화와 AMPK 활성을 상승시키는 것을 확인할 수 있었다. 지방세포 분화과정동안 키토올리고당 함유 해양심층수가 AMPK를 활성화시키고, 지방산 합성관련 유전자인 SREBP 1c, FAS, aP2의 mRNA 발현을 감소시켰다. 또한 성숙한 지방세포에서도 키토올리고당 함유 해양심층수 처리가 지방산 산화에 관여하는 CPT 1, ACO, MCAD, UCP 2의 발현과 AMPK의 활성화를 상승시킴을 확인하였다. 따라서 본 연구의 결과들은 키토올리고당 함유 해양심층수가 지방세포 분화동안 지방산 대사의 억제과정과 성숙지방세포의 지방산 산화과정의 증가과정에 해양심층수 단독처리보다 더 효과적으로 작용하며 이 과정에 AMPK의 활성화 증가가 관여할 것이라고 유추할 수 있다. 그러므로 비만 예방 및 치료 효과를 증진시키는 소재로서 키토올리고당 함유해양심층수의 활용 가능성을 확인할 수 있었다.

다국어 초록 (Multilingual Abstract)

To determine effect of deep sea water (DSW) containing chitosan oligosaccharides (COS) on fatty acids synthesis and β-oxidation, we performed GPDH activity assay and RT-PCR in differentiated or mature 3T3-L1 adipocytes with the treatment of DSW or DSW containing COS. In differentiated 3T3-L1 adipocytes, both DSW and DSW containing COS treatment inhibited GPDH activity and the expressions of genes related to fatty acids synthesis such as SREBP 1c, FAS, and aP2. On the other hands, the expressions of genes related to β-oxidation such as CPT 1, MCAD, ACO, and UCP 2 were increased in mature 3T3 L1 adipocytes. Furthermore, the phosphorylation of AMPK also was increased by DSW and DSW containing COS treatment in both differentiated and mature 3T3-L1 adipocytes. Taken together, these data suggest that DSW containing COS is more effective than DSW alone in inhibitory effect on fatty acids synthesis and stimulatory effect on β-oxidation and AMPK activation.

참고문헌 (Reference)

1 박정은, "키토올리고당 함유 해양심층수의 지질대사 억제효과" 한국키틴키토산학회 17 (17): 204-209, 2012

2 신은지, "키토올리고당 함유 해양심층수의 지방세포 분화 억제 효과" 한국키틴키토산학회 17 (17): 164-168, 2012

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