Identification of novel pathogenicity-related cellulase genes in <i>Xanthomonas oryzae</i> pv. <i>oryzae</i>

다국어 초록 (Multilingual Abstract)

<P><B>Abstract</B></P><P>Twelve genes encoding cellulases, including endo- and exoglucanases, were identified from the genomic database of <I>Xanthomonas oryzae</I> pv. <I>oryzae</I> KACC10331. The genes were amplified by polymerase chain reaction (PCR) from <I>X. oryzae</I> pv. <I>oryzae</I> KACC10859 and mutated by transposon insertion; further, marker exchange was performed with the target genes of the wild type strain. Homologous recombination events were confirmed by PCR and Southern hybridization analysis. We found that the mutant strains <I>eglXoA::Tn5</I>, <I>eglXoB</I>::<I>Tn5</I>, and <I>celXoB</I>::<I>Tn5</I> were completely virulence-deficient. In addition, mutants <I>celbXoA</I>::<I>Tn5</I>, <I>bglXoC</I>::<I>Tn5</I>, and <I>bglXoF</I>::<I>Tn5</I> showed attenuated virulence, while the virulence of other mutants was not affected.</P> <P><B>Graphical abstract</B></P><P><ce:figure id='dfig1'></ce:figure></P><P><B>Highlights</B></P><P>► Twelve cellulase genes in <I>X. oryzae</I> pv. <I>oryzae</I> genome were mutated by transposon insertion. ► Pathogenicity and complementation of the mutants were assayed on rice leaves. ► <I>eglXoA, celXoB</I>, <I>celbXoA, bglXoC</I> and <I>bglXoF</I> were identified as novel pathogenicity-related genes.</P>