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다국어 초록 (Multilingual Abstract)

This study was designed to evaluate effects of BSA, PVA, gonadotropins and follicle shell during IVM of porcine oocytes and subsequent development to the blastocyst stage after IVF. Cumulus oocyte complexes (COCs) were cultured in TCM-199 media containing 4 mg/ml BSA and 1 mg/ml PVA during IVM for 44 hr. To compare the effect of gonadotropins on oocyte maturation, COCs were cultured with FSH+LH, FSH, LH and FSH-LH-free media during IVM, respectively. Also, different number of follicle shells (0, 2, 4 and 6) was used to examine whether the presence of follicle shell in culture medium affects oocyte maturation. The percentages of fertilization and blastocyst formation, respectively, were higher in the medium containing the PVA (49.0 and 17.9%) than those containing the BSA (40.0 and 12.2%). Significantly higher rates of MII oocytes were in the presence of FSH+LH and FSH (88.6 and 85.1%) compared to other treatments (64.0 and 53.4% at LH and FSH-LH-free media). Co-culture with inverted follicle shells in 2 ml maturation medium enhanced the developmental competence of porcine oocytes. In conclusion, PVA could be used as a macromolecules instead of BSA, and FSH and follicle shell played important roles in maturation of porcine oocytes.

목차 (Table of Contents)

ABSTRACT
INTRIDUSTION
MATERIALS AND METHODS
Oocyte Collection and In Vitro maturation(IVM)
Semen Collection and Liquid Semen Processing

ABSTRACT
INTRIDUSTION
MATERIALS AND METHODS
Oocyte Collection and In Vitro maturation(IVM)
Semen Collection and Liquid Semen Processing
In Vitro Fertilization(IVF) and Culture of Oocytes
Evaluation of Oocytes and Embryos Produced In Vitro
Experimental Designs
Statistical Analysis
RESULTS
Effects of Different mTCM-199 Media on IVM
Effects of BSA and PVA and IVM
Effects of BSA and PVA on IVF and Subsequent Development
Effects of Gonadotropins on IVM
Effects of Follicle Shell Co-culture on IVM, and Embryos Development
DISCUSSION
REFERENCES

참고문헌 (Reference)

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