Potential Mechanisms Associated with Strawberry Fruit Firmness Increases Mediated by Elevated pCO2

다국어 초록 (Multilingual Abstract)

Postharvest treatment of strawberry fruit with an elevated pCO2 induces transient increases in the fruit firmness. The mechanism responsible for this firmness increase is not clearly understood. This study addressed the physiological responses of strawberry fruit to CO2 treatment to understand the factors to induce firmness increase. High CO2treatment induced modification of pectic polymers, the decrease of water-soluble pectins (WSP) and increase of chelator-soluble pectins (CSP), which are the major factors for firmness increase. The shift of WSP to CSP is related with calcium binding to WSP. The calcium binding to wall polymers was induced without changes of PME activity and methoxy content of WSP and CSP. Our results suggested that fruit firmness increase of strawberry by postharvest CO2treatment occurred primarily through pectin polymerization mediated by calcium.

참고문헌 (Reference)

1 Goto, T., "The mechanism of the increase of firmness in strawberry fruit treated with 100% CO2" 43 : 1158-1162, 1996

2 Tanaka, Y., "Studies on the CA-storage of fruits and vegetables. II. Effect of CA-storage and CO2 short term treatment on the quality of strawberry" 71-77, 1970

3 Huber, D. J., "Strawberry fruit softening: The potential roles of polyuronides and hemicelluloses" 49 : 1310-1315, 1984

4 Werner, R. A., "Rehardening of peach fruit in cold storage" 103 : 90-91, 1978

5 Werner, R.A., "Rapid changes in the firmness of peaches as influenced by temperature" 13 : 470-471, 1978

6 Pelayo, C., "Postharvest life and flavor quality of three strawberry cultivars kept at 5 degrees C in air or air + 20 kPa CO2" 27 : 171-183, 2003

7 Smith, R.B., "Postharvest carbon dioxide treatment enhances firmness of several cultivars of strawberry" 27 : 420-421, 1992

8 Harker, F. R., "Physical and mechanical changes in strawberry fruit after high carbon dioxide treatments" 19 : 139-146, 2000

9 Nunes, M. C. N., "Physical and chemical quality characteristics of strawberries after storage are reduced by a short delay to cooling" 6 : 17-28, 1995

10 Sila, D. N., "Non-enzymatic depolymerization of carrot pectin: Toward a better understanding of carrot texture during thermal processing" 71 : 1-E9, 2006

11 Blumenkrantz, N., "New method for quantitative determination of uronic acids" 54 : 484-489, 1973

12 Dangyang, K., "Mode of oxygen and carbon dioxide action on strawberry ester biosynthesis" 119 : 971-975, 1994

13 Smith, P. K., "Measurement of protein using bicinchoninic acid" 150 : 76-85, 1985

14 Suzuki, K., "Localization of calcium in the pericarp cells of tomato fruits during the development of blossom-end rot" 222 : 149-156, 2003

15 Vicente, A. R., "Influence of self-produced CO2 on postharvest life of heat-treated strawberries" 27 : 265-275, 2003

16 Siriphanich, J., "High CO2 atmosphere enhances fruit firmness during storage" 67 : 1167-1170, 1998

17 Zhang, J.Z.J., "Fruit quality, fermentation products, and activities of associated enzymes during elevated CO2 treatment of strawberry fruit at high and low temperatures" 130 : 124-130, 2005

18 Nunes, M. C. N., "Fruit maturity and storage temperature influence response of strawberries to controlled atmospheres" 127 : 836-842, 2002

19 Larsen, M., "Firmness and aroma composition of strawberries following short-term high-carbon dioxide treatments" 30 : 303-305, 1995

20 Fraeye, I., "Enzyme infusion and thermal processing of strawberries: Pectin conversions related to firmness evolution" 114 : 1371-1379, 2009

21 Maclachlan, G., "Endo-1,4-beta-glucanase, xyloglucanase, and xyloglucan endo-transglycosylase activities versus potential substrates in ripening tomatoes" 105 : 965-974, 1994

22 Ke, D., "Effects of short term exposure to low O2 and high CO2 atmospheres on quality attributes of strawberries" 56 : 50-54, 1991

23 Ueda, Y., "Effect of short-term exposure of elevated CO2 on flesh firmness and ester production of strawberry" 62 : 457-464, 1993

24 Hwang, Y. S., "Effect of postharvest CO2 application on the flesh firmness and quality in ‘'Nyoho’' strawberries" 40 : 179-182, 1999

25 Goto, T., "Effect of high carbon dioxide with short-term treatment on quality of strawberry fruits" 42 : 176-182, 1995

26 El-kazzaz, M. K., "Effect of different atmospheres on postharvest decay and quality of fresh strawberries" 73 : 282-285, 1983

27 Klavons, J.A., "Determination of methanol using alcohol oxidase and its application to methyl ester content of pectins" 34 : 597-599, 1986

28 Watkins, C. B., "Cultivar variation in response of strawberry fruit to high carbon dioxide treatments" 79 : 886-890, 1999

29 Smith, R. B., "Controlled-atmosphere storage of ‘'Redcoat’' strawberry fruit" 117 : 260-264, 1992

30 Hagerman, A.E., "Continuous spectrophotometric assay for plant pectin methyl esterase" 34 : 440-444, 1986

31 Dubois, M., "Colorimetric method for determination of sugars and related substances" 28 : 350-356, 1956

32 Matsumoto, K., "Changes in firmness and pectic polysaccharide solubility in three cultivars of strawberry fruit following short-term exposure to high pCO2" 33 : 312-328, 2010

33 Grant, G. T., "Biological interactions between polysaccharides and divalent cations: The egg-box model" 32 : 195-198, 1973

34 Morris, V.J., "Architecture of intracellular networks in plant matrices" 20 : 255-261, 2009

35 Cheng, G.W., "Alterations in structural polysaccharides during liquefaction of tomato locule tissue" 111 : 447-457, 1996