The studies were performed in order to investigate the histochemical properties and compositions of mucosubstances in the mucous cells and the matrices of the cartilages in the tongues of Chondrichthyes and Osteichthyes.
The specimens used in this st...
The studies were performed in order to investigate the histochemical properties and compositions of mucosubstances in the mucous cells and the matrices of the cartilages in the tongues of Chondrichthyes and Osteichthyes.
The specimens used in this study were obtained from the tongues of 2 species of pisces; Chondrichthyes, Scylliorhinus torazame and Isteichthyes, Anguilla japonica.
The mucosubstances were stained with alcian blue pH 2.5, alcian blue pH 1.0, alcian blue pH 0.4, alcian blue pH 2.5-PAS, alcian blue pH 1.0-PAS and aldegyde fuchsin pH 1.7-alcian blue pH 2.5 staning methods.
The results were as follow:
1. It were seen mixed with most mucous cells were contained large amount of nonsulfated acid mucins and neutral mucins, and some contained a very small amount of weak sulfated acid mucins and large amount of neutral mucins in Osteichthyes (eels), but were seen mixed with most mucous cells were contained only a large amount of neutral mucins, and some contained a very small amount of weak sulfated acid mucins and a large amount of neutral mucins in Chondrichthyes (catsharks).
2. In Osteichthyes (eels), the mocosubstances in the matrices of the lingual cartilages, were composed of acid and neutral mucins, and more of acid mucins, but were different in the regions of the matrices, i.e., the mucosubstances were contained only a large amount of strong sulfated acid mucins in lacunar walls and territorial matrices, and composed of large amount of sulfated acid mucins and a very small amount of neutral mucins in granular matrices of interterritorial matrices, but composed of a small amount of weak sulfated mucins and neutral mucins in agranular matriecs of interterritorial matrices and subperichondrial matrices. In Chondrichthyes(catsharks), the mucosubstances in the matrices of the lingual cartilages, were composed of acid and neutral mucins, but were different in the regions of the matrices, i.e., the mucosubstances were contained only a large amount of strong sulfated acid nucins in lacunar walls, but composed of large amount of strong sulfated acid mucins and neutral mucins in territorial matrices and granular matrices of interterritorial matrices, and composed of a very small amount of weak sulfated acid mucins and large amount of neutral mucins in agranular matrices and subperichondrial matrices.