Hydroxocobalamin이 내독소혈증 흰쥐 흉부 대동맥에서 Thiopental에 의한 수축 반응에 미치는 영향 = Effects of Hydroxocobalamin on Thiopental-Induced Contractile Responses of Septic Rat Thoracic Aorta

다국어 초록 (Multilingual Abstract)

Background : Endotoxins play important roles in the pathophysiologic alterations associated with sepsis so the authors examined the effects of hydroxocobalamin, Nω-nitro-L-arginine-metyl ester(L-NAME) and aminoguanidine on thiopental-induced contractile responses of lipopolysaccharide(LPS)-treated and control rat aortic rings.
Methods : Aortic ring preparation was obtained from LPS-treated(1.5mg/kg, i.p. for 18h) rats. Cumulative doses of thiopental(10-4∼3×10- 3M) were added to construct contraction response curves. Hydroxocobalamin(10-5M), L-NAME(10-6M) or aminoguanidine(10-6M) were added as NO scavenger or as NOS inhibitors. Contraction curves by cumulative doses of thiopental(10-4∼3×10-3M) were remeasured after treatment of NO scavenger or NOS inhibitors. Statistical significances(p＜0.05) were analyzed according to data characteristics by Student's t-test, paired t-test or ANOVA.
Results : The vascular responses of cumulative thiopental(10-4∼3×10 3M) administration were dose- dependent contraction treated rat was less contracted(p＜0.05). There was significant increment on vascular contraction induced by thiopental after hydroxocobalamin pretreatment in LPS-treated rat(p＜0.05), in spite of L-NAME, aminoguanidine pretreatment was failed to increase contractile forces in control and LPS-treated rats.
Conclusions : From these results, viewed from maintenance of vasomotor tone in septic state, it is suggested that hydroxocobalamin may be candidate for vasopressor during usual induction of general anesthesia. (Korean J Anesthesiol 1997; 33: 25∼32)