Glycerol-Phosphate Acyltransferase Gene Expression의 호르몬에 의한 조절 = Hormonal Regulation of Glycerol-Phosphate Acyltransferase Gene Expression

국문 초록 (Abstract)

Glycerol-phosphate acyltransferase와 7.2 kb mRNAs 모두가 간 조직에 가장 많이 존재하였다. Glycerol-phosphate acyltransferase와 7.2 kb mRNA 농도가 단식시킨 생쥐들에게 고농도 탄수화물 사료를 재급식할 경우에 상당히 증가 되었다. 성숙된 3T3-L1 adipocytes에서는 인슐린이 glycerol-phosphate acyltransferase와 7.2 kb mRNA의 농도를 2.6∼3배 증가시켰고, 반면에 dibutyryl cAMP는 mRNA 농도를 각각 50%, 80% 정도 감소시켰다. 이러한 결과들은 glycerol-phosphate acyltransferase와 7.2 kb mRNA에 대하여 인슐린에 의하여는 positive regulation(조절)이 되고 cAMP에 의하여는 negative regulation이 되는 것을 보여준다.

다국어 초록 (Multilingual Abstract)

Both glycerol-phosphate acyltransferase (GPAT) and 7.2 kb mRNAs were present at the highest level in liver. Glycerol-phosphate acyltransferase and 7.2 kb mRNA levels increased dramatically when fasted mice were refed a high carbohydrate diet. In mature 3T3-L1 adipocytes, insulin increased both glycerol-phosphate acyltransferase and 7.2 kb mRNA levels 2.6 to 3-fold while dibutyryl cAMP decreased mRNA levels by 50% and 80%, respectively. These results indicate positive regulation by insulin and negative regulation by dibutyryl cAMP of both glycerol-phosphate acyltransferase and 7.2 kb mRNA.