본 연구에서 4주령 ICR 수컷 마우스에 쇠기름과 어유에 단독 또는 0.5% CLA를 첨가한 식이로 4주동안 사육하여 혈장과 간의 지방산 조성과 D5D와 FADS2의 유전자 발현을 관찰한 결과는 다음과 같다...
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https://www.riss.kr/link?id=A76279615
2007
Korean
CLA ; beef tallow ; fish oil ; D5D ; FADS ; fatty acids composition
KCI등재,SCOPUS
학술저널
1279-1286(8쪽)
0
0
상세조회0
다운로드국문 초록 (Abstract)
본 연구에서 4주령 ICR 수컷 마우스에 쇠기름과 어유에 단독 또는 0.5% CLA를 첨가한 식이로 4주동안 사육하여 혈장과 간의 지방산 조성과 D5D와 FADS2의 유전자 발현을 관찰한 결과는 다음과 같다...
본 연구에서 4주령 ICR 수컷 마우스에 쇠기름과 어유에 단독 또는 0.5% CLA를 첨가한 식이로 4주동안 사육하여 혈장과 간의 지방산 조성과 D5D와 FADS2의 유전자 발현을 관찰한 결과는 다음과 같다. CLA는 C18:2와 경쟁적으로 지방산의 elongation과 desaturation에 참가하며, CLA의 첨가는 BTC군에서 C20:4수준을 높여주는 효과가 있었다. 또, 지방산의 β-산화에 의해 생성되기도 하는 C22:6은 BTC군에서 간의 C22:6의 함량이 BT군보다 증가하였다. D5D와 FADS2는 쇠기름섭취군(BT, BTC)에서 발현이 유의적으로 증가하였는데, 특히 D5D의 발현은 BTC군이 다른 군보다 유의적으로 증가하였다. 이에 대한 지방산 전환 또한 BTC군이 C18:2로부터 C20:4로 불포화하고 elongation하는 비율이 혈장과 간 모두에서 가장 높았다. 결론적으로 BT에 CLA를 첨가한 경우에 지방산 조성의 변화와 FADS2와 D5D의 발현을 증가시켜 지방산의 불포화도를 높여서 eicosanoids 합성과 항상성에 영향을 미치는 것으로 나타났으나 FO에 CLA첨가시에는 영향을 미치지 않는 것으로 나타났다.
다국어 초록 (Multilingual Abstract)
We investigated the effects of conjugated linoleic acid (CLA) on the fatty acid composition in the plasma and liver, and the expressions of delta-5 desaturase (D5D) and fatty acid desaturase2 (FADS2) genes in ICR male mice using two different sources ...
We investigated the effects of conjugated linoleic acid (CLA) on the fatty acid composition in the plasma and liver, and the expressions of delta-5 desaturase (D5D) and fatty acid desaturase2 (FADS2) genes in ICR male mice using two different sources of fats in the diets. The experimental groups were divided into four groups: beef tallow (BT) and fish oil (FO), BT with CLA supplementation (BTC), and FO with CLA supplementation (FOC) groups. Ten mice in each group were fed with the experimental diets for 4 weeks. All mice were fed experimental diets containing 12% of total dietary fat (w/w) either with or without 0.5%CLA (w/w). Fatty acid compositions were analyzed in the plasma and liver using gas chromatography. The levels of D5D and FADS2 expression were analyzed by RT-PCR in the liver. The results showed that CLA participates competitively with C18:2 in the elongation and desaturation processes, leading to significant increase in the levels of C20:4 and C22:6 in BTC group (p<0.05). The expression levels of D5D and FADS2 were higher in BT and BTC group than those of FO and FOC group. In particular, the expression of D5D gene was greatly upregulated in BTC group. Furthermore, the conversion ratios from C18:2 to C20:4 in the liver were higher in BTC group than those in other groups. Thus our results suggest that increased expressions of D5D and FADS2 genes may be responsible for the enhanced CLA effects on the desaturation in the BT containing saturated fatty acids rather than the FO rich in n-3 PUFA.
참고문헌 (Reference)
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1 Maritinez M., "Tissue levels of polyunsaturated fatty acids during early human development" 120 : S129-S138, 1992
2 Nakamura MT, "Structure, function, and dietary regulation of Δ6, Δ5, and Δ9 desaturases" 24 : 345-376, 2004
3 Chomczynski P, "Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction" 162 : 156-159, 1987
4 Cohen P, "Role for stearoyl-CoA desaturase-1 in leptin-mediated weight loss" 297 : 240-243, 2002
5 Liew C, "Protection of conjugated linoleic acids against 2-amino-3-methylimidazo [4, 5-f]quinoline-induced colon carcinogenesis in the F344 rat : a study of inhibitory mechanisms" 16 : 3037-3043, 1995
6 Funk CD, "Prostaglandins and leukotrienes : advances in eicosanoid biology" 294 : 1871-1875, 2001
7 Johnson DW, "Monoenoic fatty acids in human brain lipids : isomer identification and distribution" 27 : 177-180, 1992
8 Cohen P, "Leptin and the control of metabolism: role for stearoyl-CoA desaturase-1 (SCD-1)" 134 : 2455s-2463s, 2004
9 Miyazaki M, "Lack of stearoyl-CoA desaturase-1 function induces a palmitoyl-CoA delta 6 desaturase and represses the stearoyl-CoA desaturase-3 gene in the preputial glands of the mouse" 43 : 2146-2154, 2002
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11 Brenner RR, "Hormonal modulation of Δ6 and Δ5 desaturases : case of diabetes" 68 : 151-162, 2003
12 Chuang LT, "Effect of conjugated linoleic acid on fungal delta 6-desaturase activity in a transformed yeast system" 36 : 139-143, 2001
13 Park Y, "Effect of conjugated linoleic acid on body composition in mice" 32 : 853-858, 1997
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17 Belury MA, "Conjugated linoleic acid modulates hepatic lipid composition in mice" 32 : 199-204, 1997
18 Lee KN, "Conjugated linoleic acid and atherosclerosis in rabbits" 108 : 19-25, 1994
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20 DeLany JP, "Changes in body composition with conjugated linoleic acid" 19 : 487s-493s, 2000
21 Cook HW, "Alternate pathways in the desaturation and chain elongation of linolenic acid 18 : 3(n-3), in cultured glioma cells" 32 : 1265-1273, 1991
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연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2023 | 평가예정 | 해외DB학술지평가 신청대상 (해외등재 학술지 평가) | |
2020-01-01 | 평가 | 등재학술지 유지 (해외등재 학술지 평가) | |
2014-06-24 | 학회명변경 | 한글명 : 한국식품영양과학회지 -> 한국식품영양과학회영문명 : Journal of the Korean Society of Food Science and Nutrition -> The Korean Society of Food Science and Nutrition | |
2014-04-02 | 학회명변경 | 한글명 : 한국식품영양과학회 -> 한국식품영양과학회지영문명 : 미등록 -> Journal of the Korean Society of Food Science and Nutrition | |
2011-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2009-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2007-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
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학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 1.03 | 1.03 | 1.13 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
1.18 | 1.2 | 1.993 | 0.21 |