Antioxidant and Tyrosinase Inhibitory Activities of Different Parts of Guava (Psidium guajava L.)

다국어 초록 (Multilingual Abstract)

The antioxidant and the tyrosinase inhibitory activities of 4 different solvents (acetone, ethanol, methanol,and water) for preparation of extracts from guava (branch,fruit, leaf, and seed) were evaluated by measuring total phenolic contents (TPC), DPPH radical scavenging activity, ABTS radical scavenging activity, reducing power (RP), and tyrosinase inhibitory activity. The extracts of branch and leaf showed relatively higher antioxidant properties than those of fruit and seed. The highest TPC (141.28 mg/g gallic acid equivalents), DPPH radical scavenging activity (IC50=34.01 μg/mL), ABTS radical scavenging activity (IC_50=3.23 μg/mL), and RP (IC_50=75.63 μg/mL) were found in acetone extract of leaf, while water extract of seed had the lowest antioxidant activity.
The tyrosinase inhibitory activity of ethanol extract from guava leaf was 69.56%, which was the highest activity among the extracts. These results indicate that useful bioactive substances exist in the guava branch as well as leaf extracts.

참고문헌 (Reference)

1 Stangeland T, "Total antioxidant activity in 35 Ugandan fruits and vegetables" 113 : 85-91, 2009

2 Lai LS, "Studies on the antioxidative activities of hsian-tsao (Mesona procumbens Hemsl) leaf gum" 49 : 963-968, 2001

3 Oyaizu M, "Studies on product of browning reaction prepared from glucose amine" 44 : 307-315, 1986

4 Rice-Evans CA, "Structure-antioxidant activity relationships of flavonoids and phenolic acids" 20 : 933-956, 1996

5 Cvikrova M, "Soluble and cell wallbound phenolics and lignin in Ascocalyx abietina infected Norway spruces" 170 : 563-570, 2006

6 Miliauskas G, "Screening of radical scavenging activity of some medicinal and aromatic plant extracts" 85 : 231-237, 2004

7 Naczk M, "Protein precipitating capacity of phenolics of wild blueberry leaves and fruits" 96 : 640-647, 2006

8 Gutfinger T, "Polyphenols in olive oil" 58 : 966-968, 1981

9 Vinson JA, "Phenol antioxidant index: Comparative antioxidant effectiveness of red and white wines" 43 : 401-403, 1995

10 Olajide OA, "Pharmacological studies on the leaf of Psidium guajava" 70 : 25-31, 1999

11 Vanni A, "Kinetic investigations on the double enzymatic activity of the tyrosinase mushroom" 80 : 35-60, 1990

12 Lutterodt GD, "Inhibition of gastrointestinal release of acetylcholine by quercetin as a possible mode of action of Psidium guajava leaf extracts in the treatment of acute diarrhoeal disease" 25 : 235-247, 1989

13 Liang Q, "Identification of flavonoids and their glycosides by high-performance liquid chromatography with electrospray ionization mass spectrometry and with diode array ultraviolet detection" 11 : 93-101, 2005

14 Matsuo T, "Identification of (+)-gallocatechin as a bio-antimutagenic compound in Psidium guava leaves" 36 : 1027-1029, 1994

15 Jimenez-Escrig A, "Guava fruit (Psidium guajava L.) as a new source of antioxidant dietary fiber" 49 : 5489-5493, 2001

16 Moskovitz J, "Free radicals and disease" 397 : 354-359, 2002

17 Yu L, "Free radical scavenging properties of wheat extracts" 50 : 1619-1624, 2002

18 Meir S, "Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves" 43 : 1813-1817, 1995

19 Muller HE, "Detection of hydrogen peroxide produced by microorganism on an ABTS peroxidase medium" 259 : 151-158, 1985

20 Mercadante AZ, "Carotenoids from guava (Psidium guajava L): Isolation and structure elucidation" 47 : 145-151, 1999

21 Blois MS, "Antioxidant determinations by the use of a stable free radical" 181 : 1199-1200, 1958

22 Duh PD, "Antioxidant activity of burdock (Arctium lappa L.): Its scavenging effect on free radical and active oxygen" 75 : 455-461, 1998

23 Re R, "Antioxidant activity applying improved ABTS radical cation decolorization assay" 26 : 1231-1237, 1999

24 Chen HY, "Antioxidant activity and free radical-scavenging capacity of extracts from guava (Psidium guajava L.) leaves" 101 : 686-694, 2007

25 Adedapo AA, "Antioxidant activities and phenolic contents of the methanol extracts of the stems of Acokanthera oppositifolia and Adenia gummifera" 8 : 54-54, 2008

26 장인철, "Antioxidant Activity of 4 Cultivars of Persimmon Fruit" 한국식품과학회 20 (20): 71-77, 2011

27 Jaiarj P, "Anticough and antimicrobial activities of Psidium guajava Linn, leaf extract" 67 : 203-212, 1999

28 Kaur C, "Anti-oxidant activity and total phenolic content of some Asian vegetables" 37 : 153-163, 2002