아플라톡신 B1의 인간 정상세포주에 대한 ROS유도를 통한 세포사명 촉진 = Aflatoxin B1 Activates the ROS-mediated Program Cell Death Against Normal Human Cells

다국어 초록 (Multilingual Abstract)

Mycotoxin contamination of food and water is a serious global concern. Aflatoxin B1 (AFB1) is a deadly mycotoxin that contaminates both food and water bodies in the environment. AFB1 is reported to cause severe health issues, including hepatotoxicity, teratogenicity, and immunotoxicity in humans； however, the mechanistic effects against the normal human cells are not fully understood. To this aim, AFB1 contamination consequences were studied on a human normal cell skin and colon lines (HaCaT and CCD 841 CoN, respectively). Our results clearly indicate that concentrations of AFB1 greater than 10 ㎛ are toxic to HaCaT and CCD 841 CoN cells. Morphological changes of HaCaT and CCD 841 CoN cells were clearly observed after exposure to AFB1. Particularly in HaCaT cells, treatment with 50 ㎛ and 100 ㎛ AFB1 induces oxidative stress by excessive endogenous free-radical production such as ROS and NO generation. These consequences accelerate the ROS-dependent DNA damage events, which subsequently result in caspase mediated programmed cell death. Collectively, results of the current study indicate that AFB1 exposure to normal human cells triggered the programmed cell death machinery via ROS mediated pathway.