Photomixotrophic Growth of Solanum tuberosum L. in vitro with Addition and Omission of Organic Materials at Thee Initial Sucrose Levels in the Medium

국문 초록 (Abstract)

식물의 조직배양에서 가장 널리 이용되는 MS 배지 등의 무기성분은 종속영양에 적합하도록 조성되었다. 그러므로 이들 배지가 혼합영양이나 독립영양 배앙에는 부적합 할 수도 있다. 광혼합영양 미세증식에서 배지에 첨가되는 당의 수준은 낮추고 배양기의 광과 CO₂ 수준은 올리며, 광합성의 의한 탄소의 획득을 증진시키기 위해 엽록소가 있는 절편체를 이용한다. Factorial 실험에서 유기물(OM, ㎥ 배지당 각각 0.5g의 thiamine, nicotinic acid 및 pyridoxine과 100 gㆍm?³ mye-inositiol)의 첨가(+)와 생략(-) 및 세 가지 수준의 당(0, 15 및 30 ㎏ㆍm?³)이 감자 소식물체의 생장에 미치는 영향을 조사하였다. 단엽단절 절편제를 0.1×10?⁴ ㎥ MS 아가(8 ㎏ㆍm?³) 배지(증기소독 전 pH 5.80)배지가 담긴 유리 시험관(100 ㎜ × 25 ㎜)에 치상하여 직경 6㎜의 가스투과 필터(5.1 air exchangesㆍh?¹)가 부착된 투명한 필름으로 봉하여 배양하였다. 배양체는 27일간 23℃, 50% RH에서 16hㆍd?¹의 명기 동안에 cool white 형광등으로 130 μ㏖ㆍmㆍs?¹ PPFD의 광과 350-450 μ㏖ㆍ㏖?¹의 CO₂가 공급되는 배양실에서 배양되었다. 유기물이 공급된 처리(+OM)와 공급되지 않은 처리(-OM)에서 감자 소식물체의 생육은 유사하였으나 배지의 pH는 후자에서 0.2 더 낮았다. 배지에 첨가된 당의 수준이 증가할수록 건물중, % 건물율 및 경경은 증진된데 반해 신초와 뿌리의 건물중 비율, 엽면적, 건물중당 엽록소 함량 및 배지의 pH는 감소하였다. 유기물과 당 수준간의 상호작용도 신초길이와 배지 pH에서 관찰되었다. 이상의 결과로부터 광도와 CO₂ 농도를 높인 광혼합영양배양에서 감자 소식물체의 생장에는 배지에 첨가되는 당은 유익하지만 그 이외의 유기물은 첨가하지 않아도 악영향이 없다는 것은 나타낸다.

다국어 초록 (Multilingual Abstract)

The most commonly used inorganic nutrient compositions such as Murashige & Skoog medium have been optimized for heterotrophic growth. Therefore, they may not be optimal for photomixotrophic and photoautotrophic growth of plantlets. In photomixotrophic micropropagation, medium sugar level is often lowered, while light and CO₂ levels in vessel are raised, and chlorophyllous explants are used to facilitate photosynthetic carbon acquisition. In a factorial experiment effect of addition (+) and omission (-) of organic materials (OM, 0.5 gㆍm?³ each of thiamine, nicotinic acid and pyridoxine and 100 gㆍm?³ myo-inositiol) combined with three sucrose levels (0, 15, and 30 ㎏ㆍm?³) was tested on the growth of potato plantlets. Each of nodal cuttings with a leaf was cultured on 0.1 × 10?⁴ ㎥ MS agar (8 ㎏ㆍm?³) medium (pH 5.80 before autoclave) in glass test tubes (100 ㎜ × 25 ㎜) capped with a sheet of transparent film with a 6 ㎜ diameter gas permeable filter (5.1 air exchangesㆍh?¹). Cultures were maintained in a room for 27 days at 23℃, 50% RH, 350-450 μ㏖ㆍ㏖?¹ CO₂, 16 hㆍd?¹ photoperiod at 130 μ㏖ㆍmㆍs?¹ PPFD provided by white cool fluorescent lamps. Growth of potato plantlet in the +OM and -OM treatments were similar, while medium pH was 0.2 scale lower in the latter. Dry weight, % dry matter, and stem diameter enhanced, while shoot to root dry weight ratio, leaf area, chlorophyll concentration per gram dry weight, and medium pH decreased with increasing initial sucrose level. Interaction between OM and sucrose levels was observed in shoot length and medium pH. Results indicate that OM can be omitted from the medium without detrimental effect while addition of sucrose was beneficial for the photomixotrophic growth of potato plantlets under raised light and CO₂ conditions.

목차 (Table of Contents)

• Abstract
• Introduction
• Materials and Methods
• Results
• 적요
• Literature cited

참고문헌 (Reference)

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