Growth performance, carcass traits, muscle fiber characteristics and skeletal muscle mRNA abundance in hair lambs supplemented with ferulic acid = Growth performance, carcass traits, muscle fiber characteristics and skeletal muscle mRNA abundance in hair lambs supplemented with ferulic acid

다국어 초록 (Multilingual Abstract)

Ferulic acid (FA) is a phytochemical with various bioactive properties. It has recently been proposed that due to its phytogenic action it can be used as an alternative growth promoter additive to synthetic compounds. The objective of the present study was to evaluate the growth performance, carcass traits, fiber characterization and skeletal muscle gene expression on hair-lambs supplemented with two doses of FA. Thirty-two male lambs (n = 8 per treatment) were individually housed during a 32 d feeding trial to evaluate the effect of FA (300 and 600 mg d^-1) or zilpaterol hydrochloride (ZH; 6 mg d^-1) on growth performance, and then slaughtered to evaluate the effects on carcass traits, and muscle fibers morphometry from Longissimus thoracis (LT) and mRNA abundance of β2-adrenergic receptor (β₂-AR), MHC-I, MHC-IIX and IGF-I genes. FA increased final weight and average daily gain with respect to non-supplemented animals (p < 0.05). The ZH supplementation increased LT muscle area, with respect to FA doses and control (p < 0.05). Cross-sectional area (CSA) of oxidative fibers was larger with FA doses and ZH (p < 0.05). Feeding ZH increased mRNA abundance for β₂-AR compared to FA and control (p < 0.05), and expression of MHC-I was affected by FA doses and ZH (p < 0.05). Overall, FA supplementation of male hair lambs enhanced productive variables due to skeletal muscle hypertrophy caused by MHC-I up-regulation. Results suggest that FA has the potential like a growth promoter in lambs.

참고문헌 (Reference)

1 Valenzuela N, "Ácido ferúlico como promotor del crecimiento en cerdos: mecanismo y efectos sobre la deposición de tejido muscular" Centro de Investigación en Alimentación y Desarrollo 2017

2 Miller EK, "Zilpaterol hydrochloride alters abundance of β-adrenergic receptors in bovine muscle cells but has little effect on de novo fatty acid biosynthesis in bovine subcutaneous adipose tissue explants" 90 : 1317-1327, 2012

3 Young OA, "The role of insulin-like growth factor I in clenbuterol-stimulated growth in growing lambs" 73 : 3069-3077, 1995

4 Hope-Jones M, "The efficiency of electrical stimulation to counteract the negative effects of β-agonists on meat tenderness of feedlot cattle" 86 : 699-705, 2010

5 Tsiplakou E, "The effect of long term under-and over-feeding on the expression of genes related to glucose metabolism in mammary tissue of sheep" 82 : 228-235, 2015

6 Platt JP, "The effect of ferulic acid on myogenic regulators of growth in bovine satellite cells" 2012

7 Strydom PE, "The comparison of three β-agonists for growth performance, carcass characteristics and meat quality of feedlot cattle" 81 : 557-564, 2009

8 Kumar A, "Synthesis of ethyl ferulate in organic medium using celite-immobilized lipase" 102 : 2162-2167, 2011

9 Hayashi S, "Sequence of IGF-I, IGFII, and HGF expression in regenerating skeletal muscle" 122 : 427-434, 2000

10 García-González R, "Screening the activity of plants and spices for decreasing ruminal methane production in vitro" 147 : 36-52, 2008

11 Shavlakadze T, "Reconciling data from transgenic mice that overexpress IGF-I specifically in skeletal muscle" 15 : 4-18, 2005

12 Reeds PJ, "Protein and energy requirements of animals treated with β-adrenergic agonists : a discussion" 69 : 1532-1550, 1991

13 Guth L, "Procedure for the histochemical demonstration of actomyosin ATPase" 28 : 365-367, 1970

14 Busquet M, "Plant extracts affect in vitro rumen microbial fermentation" 89 : 761-771, 2006

15 Gorewit RC, "Pituitary and thyroid hormone responses of heifers after ferulic acid administration" 66 : 624-629, 1983

16 Mersmann HJ, "Overview of the effects of β-adrenergic receptor agonists on animal growth including mechanisms of action" 76 : 160-172, 1998

17 te Pas MFW, "Muscle development of livestock animals:physiology, genetics and meat quality" CABI 432-, 2004

18 Herrera RH, "Methods to accelerate muscle development, decrease fat deposits, and enhance feeding efficiency in pigs"

19 Bernabucci U, "Metabolic and hormonal acclimation to heat stress in domesticated ruminants" 4 : 1167-1183, 2010

20 Estrada-Angulo A, "Influence of level of zilpaterol chlorhydrate supplementation on growth performance and carcass characteristics of feedlot lambs" 80 : 107-110, 2008

21 Peña-Torres EF, "Hydroxycinnamic acids in animal production : pharmacokinetics, pharmacodynamics and growth promoting effects. Review" 10 : 391-415, 2019

22 Bradley J. Johnson ; Stephen B. Smith ; Ki Yong Chung, "Historical Overview of the Effect of β-Adrenergic Agonists on Beef Cattle Production" 아세아·태평양축산학회 27 (27): 757-766, 2014

23 Chen Y, "Higenamine, a dual agonist for β1-and β2-adrenergic receptors identified by screening a traditional Chinese medicine library" 85 : 738-744, 2019

24 Valadez-García KM, "Free ferulic acid supplementation of heat-stressed hair ewe lambs : oxidative status, feedlot performance, carcass traits and meat quality" 173 : 108395-, 2021

25 López-García K, "Fiber type composition of pubococcygeus and bulbospongiosus striated muscles is modified by multiparity in the rabbit" 36 : 1456-1463, 2017

26 Mizunoya W, "Fast-to-slow shift of muscle fiber-type composition by dietary apple polyphenols in rats : impact of the lowdose supplementation" 88 : 489-499, 2017

27 Anderson PT, "Encyclopedia of meat sciences" Academic Press 62-69, 2014

28 Vahedi V, "Effects of β-agonist zilpaterol hydrochloride feeding and supplementation period on growth and carcass characteristics of Lori-Bakhtiari lambs" 119 : 65-71, 2014

29 Kellermeier JD, "Effects of zilpaterol hydrochloride with or without an estrogen-trenbolone acetate terminal implant on carcass traits, retail cutout, tenderness, and muscle fiber diameter in finishing steers" 87 : 3702-3711, 2009

30 Avendaño-Reyes L, "Effects of zilpaterol hydrochloride on growth performance, carcass characteristics, and wholesale cut yield of hair-breed ewe lambs consuming feedlot diets under moderate environmental conditions" 89 : 4188-4194, 2011

31 Dávila-Ramírez JL, "Effects of zilpaterol hydrochloride and soybean oil supplementation on feedlot performance and carcass characteristics of hair-breed ram lambs under heat stress conditions" 92 : 1184-1192, 2014

32 Avendaño-Reyes L, "Effects of two β-adrenergic agonists on finishing performance, carcass characteristics, and meat quality of feedlot steers" 84 : 3259-3265, 2006

33 Macías-Cruz U, "Effects of seasonal ambient heat stress (spring vs. summer) on physiological and metabolic variables in hair sheep located in an arid region" 60 : 1279-1286, 2016

34 Eslami S, "Effects of gamma oryzanol supplementation on anthropometric measurements & muscular strength in healthy males following chronic resistance training" 139 : 857-863, 2014

35 Macías-Cruz U, "Effects of free ferulic acid on productive performance, blood metabolites, and carcass characteristics of feedlot finishing ewe lambs" 92 : 5762-5768, 2014

36 Valenzuela-Grijalva N, "Effects of ferulic acid supplementation on growth performance, carcass traits and histochemical characteristics of muscle fibers in finishing pigs" 11 : 2455-, 2021

37 Rathmann RJ, "Effects of duration of zilpaterol hydrochloride and days on the finishing diet on carcass cutability, composition, tenderness, and skeletal muscle gene expression in feedlot steers" 87 : 3686-3701, 2009

38 Wang Y, "Effects of dietary ferulic acid supplementation on growth performance and skeletal muscle fiber type conversion in weaned piglets" 101 : 5116-5123, 2021

39 Peña-Torres EF, "Effects of dietary ferulic acid on growth performance, carcass traits and meat quality of heifers" 101 : 548-554, 2021

40 Y.J. Li ; L.Y. Li ; J.L. Li ; L. Zhang ; F. Gao ; G.H. Zhou, "Effects of Dietary Supplementation with Ferulic Acid or Vitamin E Individually or in Combination on Meat Quality and Antioxidant Capacity of Finishing Pigs" 아세아·태평양축산학회 28 (28): 374-381, 2015

41 Kim YS, "Effect of the repartitioning agent cimaterol on growth, carcass and skeletal muscle characteristics in lambs" 65 : 1392-1399, 1987

42 Gonzalez JM, "Effect of ractopamine–HCl supplementation for 28 days on carcass characteristics, muscle fiber morphometrics, and whole muscle yields of six distinct muscles of the loin and round" 85 : 379-384, 2010

43 Gonzalez JM, "Effect of ractopaminehydrochloride and trenbolone acetate on longissimus muscle fiber area, diameter, and satellite cell numbers in cull beef cows" 85 : 1893-1901, 2007

44 Hahn GL, "Dynamic responses of cattle to thermal heat loads" 77 : 10-20, 1999

45 Montgomery JL, "Dietary zilpaterol hydrochloride. I. Feedlot performance and carcass traits of steers and heifers" 87 : 1374-1383, 2009

46 González-Ríos H, "Dietary supplementation of ferulic acid to steers under commercial feedlot feeding conditions improves meat quality and shelf life" 222 : 111-121, 2016

47 Paraskeuas V, "Dietary inclusion level effects of a phytogenic characterised by menthol and anethole on broiler growth performance, biochemical parameters including total antioxidant capacity and gene expression of immunerelated biomarkers" 57 : 33-41, 2016

48 Nidia Vanessa Valenzuela-Grijalva ; Humberto González-Ríos ; Araceli Pinelli-Saavedra ; Adriana Muhlia-Almazan ; David Domínguez-Díaz, "Dietary inclusion effects of phytochemicals as growth promoters in animal production" 한국축산학회 59 (59): 1-17, 2017

49 Macías-Cruz U, "Crecimiento y características de canal en corderas tratadas con clorhidrato de zilpaterol durante primavera y verano" 4 : 1-12, 2013

50 Beermann DH, "Cimaterol-induced muscle hypertrophy and altered endocrine status in lambs" 65 : 1514-1524, 1987

51 Zhao Z, "Chemistry, natural sources, dietary intake and pharmacokinetic properties of ferulic acid : a review" 109 : 691-702, 2008

52 Karlström K, "Capillary supply in relation to muscle metabolic profile and cardiocirculatory parameters" 3 : 239-244, 1991

53 Beermann DH, "Beta-adrenergic receptor agonist modulation of skeletal muscle growth" 80 : E18-E23, 2002

54 Mach N, "Association between animal, transportation, slaughterhouse practices, and meat pH in beef" 78 : 232-238, 2008

55 M.T. Lee ; W.C. Lin ; B. Yu ; T.T. Lee, "Antioxidant capacity of phytochemicals and their potential effects on oxidative status in animals — A review" 아세아·태평양축산학회 30 (30): 299-308, 2017

56 Kim KM, "Anti-stress and anti-fatigue effects of fermented rice bran" 65 : 2294-2296, 2001

57 Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method" 25 : 402-408, 2001

58 Baxa TJ, "Additive effects of a steroidal implant and zilpaterol hydrochloride on feedlot performance, carcass characteristics, and skeletal muscle messenger ribonucleic acid abundance in finishing steers" 88 : 330-337, 2010

59 Nachlas MM, "A histochemical method for the demonstration of diphosphopyridine nucleotide diaphorase" 4 : 29-38, 1958