세포질로의 siRNA 전달을 위해, 본 연구는 양이온성의 키토산과 음이온성인 tripolyphosphate(TPP)의 이온결합을 이용하여 유전자 나노전달체(Chi-TPP-siRNA)를 제조하였다. 키토산의 분자량 및 키토산...
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https://www.riss.kr/link?id=A107873239
2021
Korean
KCI등재,SCIE,SCOPUS
학술저널
697-703(7쪽)
0
0
상세조회0
다운로드국문 초록 (Abstract)
세포질로의 siRNA 전달을 위해, 본 연구는 양이온성의 키토산과 음이온성인 tripolyphosphate(TPP)의 이온결합을 이용하여 유전자 나노전달체(Chi-TPP-siRNA)를 제조하였다. 키토산의 분자량 및 키토산...
세포질로의 siRNA 전달을 위해, 본 연구는 양이온성의 키토산과 음이온성인 tripolyphosphate(TPP)의 이온결합을 이용하여 유전자 나노전달체(Chi-TPP-siRNA)를 제조하였다. 키토산의 분자량 및 키토산과 TPP의 몰비에 따른 나노입자(Chi-TPP)의 물리화학적 특성을 동적광산란기(DLS)와 전자 현미경(EM)으로 확인하였다. siRNA 담지 효과는 전기영동으로 확인하였다. MDA-MB-231에 대한 나노전달체의 생물학적 특성을 공초점현미경으로 확인하였다. 모델 siRNA 담지 Chi-TPP-siRNA 나노전달체는 직경과 다분산도가 각각 223.5 nm, 0.229인 구형으로 형성되었다. 나노전달체 처리군(500 μg/mL)에서의 세포 생존율은 88% 이상이었다. siGFP를 담지한 나노전달체(Chi-TPP-siGFP)는 48.1%의 green fluorescent protein(GFP) 유전자 억제 효율을 보였다. 결과적으로 생체친화성의 Chi-TPP-siRNA 나노전달체는 다양한 유전자 치료에 이용될 수 있다.
다국어 초록 (Multilingual Abstract)
This study is to prepare chitosan-based siRNA nano-carriers (Chi-TPP-siRNA) using ionic gelation between cationic chitosan and anionic tripolyphosphate (TPP) for delivery of siRNA to cytoplasm. We studied the effect of the molecular weight of chitosan...
This study is to prepare chitosan-based siRNA nano-carriers (Chi-TPP-siRNA) using ionic gelation between cationic chitosan and anionic tripolyphosphate (TPP) for delivery of siRNA to cytoplasm. We studied the effect of the molecular weight of chitosan and the mole ratio of chitosan/TPP on the physicochemical properties of Chi-TPP nanoparticles by using dynamic light scattering and electron microscopy. The siRNA encapsulation was evaluated by using electrophoresis. The biological properties of Chi-TPP-siRNA on MDA-MB-231 cells were evaluated by using confocal laser microscopy. The resulting Chi-TPP-siRNAs, using control siRNA, were formed in sphere shape. The diameter and the PdI value of Chi-TPP-siRNA were 223.5 nm and 0.229, respectively. The cell viability of Chi-TPP-siRNA at the concentration of 500 μg/mL was above 88%. Green fluorescent protein (GFP) silencing effect of Chi-TPP-siGFP was evaluated to be 48.1%. In conclusion, the biocompatible nano-carrier, Chi-TPP-siRNA, could be used in various gene therapy.
참고문헌 (Reference)
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1 홍웅길, "화장품 보존제로 응용하기 위한 트로폴론이 담지된 키토산 나노입자의 제조 및 특성" 한국고분자학회 44 (44): 535-541, 2020
2 정소윤, "카테콜기로 치환된 글리콜 키토산의 젤화거동 및 자가치유능" 한국고분자학회 44 (44): 225-232, 2020
3 전가영, "인슐린을 함유한 키토산-폴리카프로락톤 복합 나노섬유 시트형 창상피복재의 특성평가" 한국고분자학회 44 (44): 549-558, 2020
4 Dhrisya Chenthamara, "Therapeutic efficacy of nanoparticles and routes of administration" 한국생체재료학회 23 (23): 167-195, 2019
5 Kim, C. H., "Synthesis of Chitosan Derivatives with Quaternary Ammonium Salt and Their Antibacterial Activity" 38 : 387-393, 1997
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7 Chun Ho Kim, "Synthesis and Antibacterial Activity of Quaternized Chitosan Derivatives Having Different Methylene Spacers" 한국공업화학회 8 (8): 71-76, 2002
8 Park, S. J., "Substance‐P and Transforming Growth Factor‐β in Chitosan Microparticle‐pluronic Hydrogel Accelerates Regenerative Wound Repair of Skin Injury by Local Ionizing Radiation" 12 : 890-896, 2018
9 Raja, M. A. G., "Stability, Intracellular Delivery, and Release of siRNA from Chitosan Nanoparticles Using Different Cross-linkers" 10 : e0128963-, 2015
10 Palmeira-de-Oliveira, R., "Sodium Tripolyphosphate: An Excipient with Intrinsic in vitro anti-Candida Activity" 421 : 130-134, 2011
11 Ragelle, H., "SiRNA Delivery Methods" Springer 143-150, 2016
12 양희망, "Screening of Polymer Additives in Poloxamer 407 Hydrogel Formulations for Intravesical Instillation: Evaluation of Mechanical Properties, Gel-forming Capacity, and Drug Release" 한국고분자학회 44 (44): 817-826, 2020
13 Yong Kiel Sung, "Recent advances in polymeric drug delivery systems" 한국생체재료학회 24 (24): 150-161, 2020
14 Wang, J. J., "Recent Advances of Chitosan Nanoparticles as Drug Carriers" 6 : 765-, 2011
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Comparative Study of Tribological Properties of Multilayers Laminate Nanocomposites
플루페나민산이 담지된 키토산 나노입자의 제조 및 항암 효과
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2023 | 평가예정 | 해외DB학술지평가 신청대상 (해외등재 학술지 평가) | |
2020-01-01 | 평가 | 등재학술지 유지 (해외등재 학술지 평가) | |
2012-06-04 | 학술지명변경 | 외국어명 : 미등록 -> POLYMER(KOREA) | |
2010-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2008-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2006-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2004-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2001-07-01 | 평가 | 등재학술지 선정 (등재후보2차) | |
1999-01-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.58 | 0.47 | 0.5 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.45 | 0.43 | 0.401 | 0.13 |