네발나비과 나비류의 계대사육법 체계확립 = Establishment of the Successive Rearing System of Brush-footed Butterflies (Lepidoptera: Nymphalidae)

국문 초록 (Abstract)

곤충관의 연중 전시용으로서 붉은 색 계통의 화려한 나비로서 공급하기 위하여 네발나비류의 계대사육법 체계확립을 위해 일련의 시험을 수행하였다. 먼저 실내 제대사육용 인공사료를 개발하기 위해 3종의 사료를 공시한 결과 종에 따라 선호하는 사료의 종류가 달랐으나 Bu사료가 대체로 사육 성적이 양호하였으며 사료 중 기주잎 분말은 냉동 건조한 것이 좋았으며 C/N율은 1:1이 가장 양호한 결과로 나타났다. 또한 발육단계 별 냉장가능기간을 조사한 결과 알의 경우 $5^{\circ}C$에서는 3일간, $15^{\circ}C$에서는 5일간 저장이 가능하였고 번데기의 경우에는 저온에 오래 보관할수록 기형 나비의 출현율이 높아 $15^{\circ}C$에서 최대 15일간 냉장 가능한 것으로 판단되었다. 나비의 장기 저온보호시험을 통해 냉장전의 흡밀기간, 냉장 온도에는 관계없이 법 장기간이 생존을, 산란전기간 및 산란수에 영향하였으며 60일까지 냉장이 가능한 것으로 나타났다. 네발나비는 기주식물의 자극없이는 산란하지 않아 페트리 접시를 이용한 간이 인공산란용 키트를 개발하여 기주식물(한삼덩굴)잎의 에테르 물추출물을 처리한 결과 암컷 1마리 당 평균 $278{\pm}27$개를 산란하였다. 이러한 결과들을 종합하여 네발나비류의 연중 실내제대사육 체계도를 완성하였다.

다국어 초록 (Multilingual Abstract)

In order to establish the successive rearing system brush-footed butterflies (Lepidoptera : Nymphalidae) were reared in a room. Artificial diets were developed for a year-round rearing. Bu-diet was best to rear these butterflies among 3 kinds of diet used. The freeze-dried host plant leaf powder in diet was better than heat-dried one $(60^{\circ}C)$ in the growth of larvae. The rearing results were best in the diet C/N ratio was 1:1. The 24-hrs old eggs could be stored for 5 days at $15^{\circ}C$ or for 3 days at $5^{\circ}C$ and showed 75% of hatchability. On the other hand, pupae could be stored for maximum 15 days at $15^{\circ}C$ because the emergence of abnormal adults appeared much more as the cold storage period got longer. And the adult was able to be stored until 60 days at refrigerator without relation of nectar-sucking period before cold-storage and storage temperature. Also a simple artificial ovipositing kit was devised by ${\Phi}9$ cm of petri-dish and a female oviposited $278{\pm}27$ of eggs with adding the ether extract of host plant to this kit. The systematic successive rearing method of brush-footed butterflies in a room was completed.

참고문헌 (Reference)

1 "Relation between accumulation of secretory fluid in the accessory gland of the female genital organ and activity of the corpus allatum in Polygonia c-aureum L" 53-82 58, 1973

2 "Oviposition behavior of the sweet potato hornworm, Agrius convolvuli(Lepidoptera: Sphingidae), as analysed using an artificial leaf" 33 : 525-534, 1998

3 "Neuroendocrine regulation of the development of seasonal morphs in the Asian comma butterfly Difference in activity of summer-morph-producing hormone from brain-extracts of the long-day pupae" 145-152, 1988

4 "Hormonal control of seasonal morphs by the timing of ecdysteroid release in Araschnia levana L" 823-829, 1987

5 "Hormonal control of seasonal morph determination in the swallowtail butterfly" 669-674, 1985

6 "Hormonal control of seasonal morph determination in the small copper butterfly" 701-706, 1985

7 "Establishment of the successive rearing method of cabbage butterfly, Pieris rapae L. in a room condition." 40 (40): 131-136, 2001

8 "Day length as the main factor of seasonal form determination in Polygonia c-aureum" 77-83, 1963

9 "112-115 In Insect's Life in Korea" Korea university 1988