Infectious in vivo Transcripts from a Full-length Clone of Soybean mosaic virus Strain G5H

다국어 초록 (Multilingual Abstract)

An infectious full-length clone of Soybean mosaic virus
(SMV) strain G5H was constructed under the control of
the cauliflower mosaic virus 35S promoter. The cloned
SMV G5H established infections upon simple rubinoculation
of soybean leaves with intact plasmid DNA.
We demonstrated that this SMV G5H infectious DNA
clone caused typical characteristic symptoms and virulence
of SMV strain G5H in twelve tested soybean
cultivars. Soybean cultivars Lee74, Somyungkong and
Sowonkong developed systemic mosaic symptom while
Kwanggyo, Taekwangkong, Hwangkeumkong and
Geumjeongkong-1 showed systemic necrosis. In contrast,
Geumjeongkong-2, Jinpumkong-2, L29, V94-5152
and Ogden showed resistant response against SMVG5H
infection. We also determined full-length sequence
of cloned SMV-G5H. The phyogenetic analyses reveal
that SMV-G5H is most closely related to SMV-G5, and
support that SMV-G5H might be derived from SMVG5
by recombination rather than mutation.

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