돌산갓의 원예적 특성 및 RAPD에 의한 유연관계 분석 = Analysis of Genetic Similarity of Dolsan Leaf Mustard Based on Several Horticultural Traits and RAPD

국문 초록 (Abstract)

김치 제조용 갓 24종의 원예적특성을 조사하였으며, 주성분 분석을 통하여 유사성 정도를 분석하였다. 주성분 분석결과 생체중, 건물중, 엽장, 엽수의 누적기여도가 높게 나타났으며 이 4가지 요인만으로도 24종의 유연관계를 분석할 수 있었다. 또한 수량면에서 가장 우수한 종으로‘Goheung dongil’을 선발하였다. RAPD분석에 의해 24종간의 DNA다형성 정도와 유전적 유연관계를 검토하였다. DNA 다형성을 나타내는 4종의 primer를 선발하였으며, 표지 12개를 선발하여 계통 및 품종 식별의 단서를 마련하였다. UPGMA 분석에 의해 갓 24종은 5개의 군으로 분류되었으며, 각 수집종간의 유전적 유사도를 확인 할 수 있었다. 그러나 양적형질 분석에서 유전적 거리가 가깝게 나타난 종들이 반드시 RAPD 분석결과와 일치하지는 않았다. 실험결과 얻어진 양적형질, 형태적 특징 및 RAPD분석에 의한 유사도 결과는 세포질 웅성불임성을 이용한 F1 생산의 기초자료로 이용할 것이다.

다국어 초록 (Multilingual Abstract)

The characteristics of 24 leaf mustard (Brassia juncea) accessions were analyzed using quantitative characters and principal component analysis. Cumulative contribution of fresh weight, dry weight, leaf length and number of leaf showed high volume by the principal component analysis. The genetic relationship among 24 accessions can be analized by these four characters. In addition the ‘Goheung dongil’ was selected for greatest accessions in only yield side. RAPD marker was used to analysis the DNA polymorphism and genectic relationship among 24 accessions. Four random primers were selected by which 12 polymorphic bands. Based on the UPGMA cluster analysis 24 accessions were separated into five groups. However, the results that showed very close relationship in quantitative character analysis were not constantly agreed with RAPD analysis. The results of quantitative analysis and application of the 12 RAPD markers are available for the F1 hybrid production by using cytoplasmic male sterility of leaf mustard.

목차 (Table of Contents)

• Abstract
• 서언
• 재료 및 방법
• 결과 및 고찰
• 초록
• 인용문헌

참고문헌 (Reference)

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