언체 위(胃)의 해부학적 구획에 따른 그렐린 mRNA 발현의 정량적 분석 = Quantitative evaluation of the ghrelin mRNA expression in the anatomic subdivisions of human stomach

다국어 초록 (Multilingual Abstract)

Purpose: Ghrelin is a endogenous ligand of the GH secretagogure (GHS) receptor mainly produced by the stomach. However, the ghrelin production capacity according to anatomic subdivisions of human stomach is not yet well established. The purpose of this study was to investigate ghrelin mRNA expression in the anatomic subdivisions of normal gastric tissue as well as in cancer tissue.
Subject and Methods: Tissue samples were obtained from 16 individuals who underwent total gastrectomy for stomach cancer. The normal gastric tissues were harvested from the upper, middle, lower portion of stomach and cancer tissues from tumor mass, respectively. Total RNA was reverse transcribed with random hexamer primers and ghrelin mRNA was amplified by real time RT-PCR using specific primers and hybridization probes.
Results: In normal gastric tissue, ghrelin mRNA was expressed and in cancer tissue, also. Mean ghrelin mRNA expression of upper, middle, lower portion of stomach and cancer tissues, expressed as the ratio of ghrelin mRNA to β-actin concentration, were 0.78 (range 0.041-3.88),0.20(range 01.28), 0.07 (range 0-0.67), 0.32 (range 0-3.67), respectively. The expression of ghrelin mRNA in upper portion of stomach was significantly higher than that in the lower portion of stomach (P<0.05). There was no correlation between ghrelin mRNA expression in upper portion of stomach and clinical parameters, such as age, sex and BMI.
Conclusions: Ghrelin mRNA was expressed in cancer tissue as well as normal gastric tissue. The expression of ghrelin mRNA in the anatomic subdivisions of normal gastric tissue was higher in upper portion compared to the other portion of stomach. These findings provide clues to additional, as yet undefined, gastric neuroendocrine functions of this novel gastrointestinal hormone