The Pluripotent Status of Porcine Embryonic Stem Cell Lines Derived from Various Origins

다국어 초록 (Multilingual Abstract)

The pluripotent stem cells can be divided to naïve and primed state according to their pluripotent state. In this study, total 7 primed ESC lines were derived from porcine embryos of various origins, including in vitro fertilized (IVF), parthenogenetic activation (PA) and nuclear transfer (iPS-NT) from a donor cell with induced pluripotent stem cells (iPSC). To characterize ESCs, we performed several approaches including morphological observation, intensive alkaline phosphatase activity, the strongly pluripotency-associated genes such as POU5F1 (formerly known as OCT4), Sox2 and NANOG, immunocytochemistry analysis, the three germ layer associated genes such as AFP (edndoderm), DESMIN (mesoderm), CRABP2 (ectoderm), karyotyping and embryoid body formation. Furthermore, we investigated the XIST gene expression from all female cell lines. Establishment of porcine naïve ESCs, especially iPS-NT ESCs, will make possible sophisticated genetic manipulation to create ideal animal models for pre-clinical research and studies of human diseases. This study will help to approach the goal for establishing naive pluripotent stem cells in pigs.