P. gingivalis에 대한 피톤치드의 항균효과 = Effect of Phytoncide on Porphyromonas gingivalis

다국어 초록 (Multilingual Abstract)

Trees emit phytoncide into atmosphere to protect them from predation. Phytoncide from different trees has its own unique fragrance that is referred to as forest bath. Phytoncide, which is essential oil of trees, has microbicidal, insecticidal, acaricidal, and deodorizing effect. The present study was performed to examine the effect of phytoncide on Porphyromonas gingivalis, which is one of the most important causative agents of periodontitis and halitosis. P. gingivalis 2561 was incubated with or without phytoncide extracted from Hinoki (Chamaecyparis obtusa Sieb. et Zucc.; Japanese cypress) and then changes were observed in its cell viability, antibiotic sensitivity, morphology, and biochemical/molecular biological pattern. The results were as follows: 1. The phytoncide appeared to have a strong antibacterial effect on P. gingivalis. MIC of phytoncide for the bacterium was determined to be 0.008%. The antibacterial effect was attributed to bactericidal activity against P. gingivalis. It almost completely suppressed the bacterial cell viability (>99.9%) at the concentration of 0.01%, which is the MBC for the bacterium. 2. The phytoncide failed to enhance the bacterial susceptibility to ampicillin, cefotaxime, penicillin, and tetracycline but did increase the susceptibility to amoxicillin. 3. Numbers of electron dense granules, ghost cell, and vesicles increased with increasing concentration of the phytoncide, 4. RT-PCR analysis revealed that expression of superoxide dismutase was increased in the bacterium incubated with the phytoncide. 5. No distinct difference in protein profile between the bacterium incubated with or without the phytoncide was observed as determined by SDS-PAGE and immunoblot. Overall results suggest that the phytoncide is a strong antibacterial agent that has a bactericidal action against P. gingivalis. The phytoncide does not seem to affect much the profile of the major outer membrane proteins but interferes with antioxidant activity of the bacterium. Along with this, yet unknown mechanism may cause changes in cell morphology and eventually cell death.

참고문헌 (Reference)

1 "편백정유의 항균효과" 29 : 253-257, 2001

2 "침엽수 침엽 정유의 방향성 이용적성" 49 : 177-179, 1994

3 "침엽수 수엽 정유의 항균성에 관한 연구" 13 : 71-79, 1993

4 "several other species of Pseudomonas and Escherichia coli to hydrophobic drugs" Vaara M 1741-1747,

5 "monitoring of subgingival Bacteroides gingivalis and Actinobacillus actinomycetemcomitans in the management of advanced periodontitis" 2 : 382-388, 1988

6 "The superoxide dismutase-encoding gene of the obligately anaerobic bacterium Bacteroides gingivalis" 96 : 149-150, 1990

7 "The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil)" 88 : 170-175, 2000

8 "The antimicrobial activity of essential oils and essential oil components towards oral bacteria" 9 : 202-208, 1994

9 "Requirement for intercellular adhesion molecule 1 and caveolae in invasion of human oral epithelial cells by Porphyromonas gingivalis" 73 : 6290-6298, 2005

10 "Release and activation of human neutrophil matrix metallo- and serine proteinases during phagocytosis of Fusobacterium nucleatum, Porphyromonas gingivalis and Treponema denticola" 24 : 237-248, 1997

11 "Rapid viability loss on exposure to air in a superoxide dismutase-deficient mutant of Porphyromonas gingivalis" 176 : 1939-1943, 1994

12 "Production and origin of oral malodor. A review of mechanisms and methods of analysis." 48 : 13-20, 1997

13 "Porphyromonas gingivalis에 대한 polyphosphate의 항균효과" 34 : 285-301, 1999

14 "Plant products as antimicrobial agents" 12 : 564-582, 1999

15 "Oral Malodor: philosophical and practical aspects" 63 : 196-201, 1997

16 "Molecular cloning and sequencing of the gene encoding the fimbrial subunit protein of Bacteroides gingivalis" 170 : 1658-1665, 1988

17 "Microbiota of periodontal pockets losing crestal alveolar bone" 11 : 279-291, 1984

18 "Mechanism of action of Spanish oregano, Chinese cinnamon, and savory essential oils against cell membranes and walls of Escherichia coli O157:H7 and Listeria monocytogenes" 69 : 1046-1055, 2006

19 "Mechanism of action of Melaleuca alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage, and salt tolerance assay and electron microscopy" 46 : 1914-1920, 2002

20 "Manual of Clinical Microbiology American Society for Microbiology" 1000-1008, 1985

21 "Life below the gum line: pathogenic mechanisms of Porphyromonas gingivalis" 62 : 1244-1263, 1998

22 "Interactions between black- pigmented Gram-negative anaerobes and other species which may be important in disease development" 6 : 173-178, 1993

23 "Inorganic polyphosphate supports resistance and survival of stationary-phase Escherichia coli" 178 : 1394-1400, 1996

24 "Five essential oils from aromatic plants of Cameroon: their antibacterial activity and ability to permeabilize the cytoplasmic membrane of Listeria innocua examined by flow cytometry" 39 : 395-400, 2004

25 "Essential oils and aromatherapy:their modern role in healing" 117 : 324-329, 1977

26 "Effects of various growth conditions in a chemostat on expression of virulence factors in Porphyromonas gingivalis" 72 : 3458-3467, 2006

27 "Effects of sublethal exposure to an antiseptic mouthrinse on representative plaque bacteria" 23 : 444-451, 1996

28 "Correlation between oral malodor and periodontal bacteria" 4 : 679-683, 2002

29 "Complete genome sequence of the oral pathogenic Bacterium Porphyromonas gingivalis strain W83" 185 : 5591-5601, 2003

30 "Complementary therapies in hospice care: touch with oils- a pertinent part of holistic care" 14 : 42-44, 1997

31 "Comparison of the microbiota of supra- and subgingival plaque in health and periodontitis" 27 : 648-657, 2000

32 "Clinical and microbiological effects of different antimicrobials on generalized aggressive periodontitis" 33 : 254-264, 2006

33 "Biology of asaccharolytic black-pigmented Bacteroides species" 52 : 134-152, 1988

34 "Analysis of major virulence factors in Porphyromonas gingivalis under various culture temperatures using specific antibodies" 48 : 561-569, 2004

35 "Alleochemics:chemical interactions between species" 171 : 757-770, 1971

36 "Allelopathy as a factor in ecological processes" 18 : 348-357, 1969

37 "Advanced aromatherapy" Healing Arts Press 255-286, 1995

38 "Activation and novel processing of matrix metalloproteinases by a thiol-proteinase from the oral anaerobe Porphyromonas gingivalis" 76 : 1260-1270, 1997

39 "A rapid method of evaluating permeabilizing activity against Pseudomonas aeruginosa" 17 : 149-151, 1993

40 "A comparison of the antibacterial efficacies of essential oils against oral pathogens" 19 : 61-64, 2004