Shigellosis is one of the most common infectious diseases in Korea. Although the prevalence of the disease tends to be decreasing, it still outbreaks sporadically and endemically in Korea. As the control of the disease depends on the eradication of th...
Shigellosis is one of the most common infectious diseases in Korea. Although the prevalence of the disease tends to be decreasing, it still outbreaks sporadically and endemically in Korea. As the control of the disease depends on the eradication of the carriers, it is very important to detect carriers by bacterial culture. As ordinary culture media for Shigella, S-S agar medium, contains several inhibitors of bacterial growth and the rate of colony formation is very low, it is necessary to develop new sensitive media for the detection of the bacteria from carriers.
This study was performed to investigate the effect of sodium citrate for the growth of Shigella spp. in peptone agar and BHI agar medium and to develop a sensitive culture medium for Shigella spp. The results were as follows :
1. The growth of all Shigella spp. was inhibited in the peptone agar medium containing sodium citrate. The higher the concentration of the sodium citrate, the more the growth of the Shigella spp. was inhibited.
2. The growth of all Shigella spp. was also inhibited in the BHI agar medium containing sodium citrate.
3. S-S agar medium was not suitable for an isolation medium of Shigella spp.
Above results showed that sodium citrate is not suitable component of the sensitive detection medium for Shigella spp. and the studies on the development of new sensitive detection medium of Shigella spp. should be further carried out.