Eugenol, citral, and hexanal, alone or in combination with heat, affect viability, biofilm formation, and swarming on Shiga-toxin-producing Escherichia coli

다국어 초록 (Multilingual Abstract)

Shiga-toxin-producing Escherichia coli strainsare pathogenic for humans and cause mild to severe illnesses.
In this study, the antimicrobial effect of citral,eugenol, and hexanal in combination with heat shock (HS)was evaluated in terms of the growth, biofilm formation,swarming, and expression of virulence genes of STECserotypes (O157:H7, O103, O111, and O26). Eugenol wasthe most effective compound against the growth of E. colistrains (MBC = 0.58 to 0.73 mg/mL), followed by citral(MBC = 0.86 to 1.26 mg/mL) and hexanal (MBC = 2.24to 2.52 mg/mL). Biofilm formation and swarming motilityhave great variability between STEC strains. Naturalcompounds—alone or combined with HS—inhibited biofilmformation; however, swarming motility was inducedby most treatments. The expression of the studied genesduring biofilm formation and swarming under naturalantimicrobials was affected but not in a uniform pattern.
These treatments could be used to control contamination ofSTEC and inhibit biofilm formation.

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