군산인근해역에서 분리동정된 Vibrio 속의 특성과 해수에서의 생존 = Characteristics and Survival of Genus Vibrio Isolated in the Intertidal Zone of the Yellow Sea near Kunsan

다국어 초록 (Multilingual Abstract)

To investigate the population dynamics and survival of Genus Vibrio, population densities of aerobic saprophytic bacteria and Vibrio groups were measured 4 times in the intertidal waters of the Yellow Sea near Kunsan from November, 1997 to June, 1998. The distribution of heterotrophic bacteria during the survey periods by plate count and direct count method ranged from 1.2±0.6×10 exp (3) to 2.0±1.5×10 exp (4) CFU ml^-1 and from 6.0±4.0×10 exp (5) to 1.9±1.5×10 exp (7) cells ml^-1, respectively. Vibrio groups were distributed in the range of 1×10 and 6±2.2×10 exp (2) CFU ml^-1. The proportion of Vibrio groups to total heterotrophic bacteria was between 0.1 and 6% during the survey periods. A total of 51 isolates was obtained from TCBS agar plates and identified to species level by Biolog Identification System^™. As a result, dominant genera were V. mediterranei, V. anguillarum, V. metschnikovii, and V. parahaemolyticus, and isolates were clustered into 26 groups based on the relatedness of average linkage clustering method at 70% level. As for the susceptibility of 51 isolates to 7 kinds of antibacterial agents (gentamicin, ampicillin, chloramphenicol, streptomycin, kanamycin, tetracycline, carbenicillin), 96% of isolates showed high resistance to more than one antibiotics and 65% of isolates contained a plasmid, of which size was observed greater than 12kb. The number of cells of 3 tested strains (V. anguillarum, V. vulnificus, and V. metschnikovii) in filtered aged seawater decreased by approximately 1 to 5 orders of magnitude during 30-d incubation. In most cases, the numbers of cells decreased rapidly until day 3,then decreased slowly by day 30. The number of cells incubated at 15℃ showed higher survival than those at 4℃ and 25℃. These results may be considered for the basic supporting data in the risk assessment of vibriosis in summer. [Vibrio, distribution, Biolog Identification system, resistance to antibiotics, survival in seawater].