Human fibroblast Interferon cDNA of which the signal sequence was elimiated by the partial digestion of the restriction enzyme AluI was inserted into the E. Coli expression vector, pkk223-3 which has tac promoter and transcription terminator region, r...
Human fibroblast Interferon cDNA of which the signal sequence was elimiated by the partial digestion of the restriction enzyme AluI was inserted into the E. Coli expression vector, pkk223-3 which has tac promoter and transcription terminator region, rrnBT₁T₂ and into PLc2833 which has λpL promoter. The former was transformed to JM105 and JM103 and the latter was transformed to NR69 and M5248. The orientation of the IFN-β toward promoter was ascertained by restriction enzyme cutting. Expression of IFN-βgene of pkk223-3-IFN-βin E. Coli was induced by IPTG and the of PLc2833-IFN-β was induced by heat-shock. The induced E. Coli lysates were analysed by using SDS-PAGE.