Inheritance of Anthracnose (Colletotrichum acutatum) Resistance in Capsicum Using Interspecific Hybridization = 종간 교잡을 이용한 고추 탄저병 저항성 유전 분석

국문 초록 (Abstract)

지금까지 Colletotrichum acutatum에 의해서 발생하는 고추(Capsicum spp.) 탄저병에 대한 안정적인 저항성 유전자원은 근연종인 Capsicum baccatum과 C. chinense에서만 동정되었다. C. baccatum var. PBC81의 탄저병 저항성을 C. annuum var. Matikas로 도입하기 위한 종간 교잡 및 배구제 방법이 사용되었다. 종간 교잡 F₁ 식물체들은 모두 완전한 화분 불임이어서 재배종인 ‘Matikas’를 화분친으로 사용하여 집중적인 여교잡을 수행한 결과 BC₁F₁ 집단을 육성할 수 있었다. 얻어진 수백 개의 BC₁F₁ 개체 중에서 탄저병 접종에 사용할 수 있는 다수의 과실이 달린 88개체만이 탄저병 저항성 유전 분석에 사용되었다. BC₁F₁ 집단의 저항성과 이병성의 분리비는 1:1 또는 3:1의 멘델식 분리비에 적합하지 않았으며, 집단의 빈도 분포는 저항성에서 이병성으로 연속분포를 보였다. 탄저병 저항성에 대한 광의의 유전력 측정을 위하여 selection differential(i)과 genetic gain(ΔG)을 이용하였고 공식 h²= ΔG/i에 의해 추정한 유전력은 약 0.61로 측정되었다. 이러한 결과는 C. baccatum var. PBC81의 탄저병 저항성이 몇 개의 유전자에 의해서 조절되는지는 알 수 없지만 다수의 유전자가 관여하는 양적유전형질이라는 것을 나타내는 것이다.

다국어 초록 (Multilingual Abstract)

Reliable genetic resources resistant to anthracnose, caused by Colletotrichum acutatum, in Capsicum have only been found in certain Capsicum species, e.g., Capsicum baccatum and C. chinense. Interspecific hybridization using embryo rescue was used to introgress anthracnose resistance from C. baccatum var. PBC81 into C. annuum var. Matikas. Because the interspecific hybrids were completely pollen-sterile, an interspecific BC₁F₁ as the first segregating population was raised through intensive backcrosses in which Matikas was used as the male parent. Among the hundreds of BC₁F₁ progeny, 88 plants setting many fruits were used to study inheritance of anthracnose resistance. The segregation of resistance to susceptibility in the BC₁F₁ population did not fit expected Mendelian ratios such as 1:1 or 3:1, and the frequency distribution of progenies varied continuously from resistant to susceptible. To estimate broad sense heritability of resistance, we used a real method of selection differential (i) and genetic gain (ΔG). According to the equation h²= ΔG/i, heritability was estimated as about 0.61. This result suggests that resistance of C. baccatum var. PBC81 can be controlled quantitatively, although we do not know yet how many genes are associated with the resistance.

목차 (Table of Contents)

• Abstract
• Introduction
• Materials and Methods
• Results and Discussion
• Literature Cited
• 초록

참고문헌 (Reference)

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