The Efficiency of Deer Antler Herbal Acupuncture on Modulation and Prevention of IL-1 Mediated Activation in Rat Chondrocytes at a Receptor Level

다국어 초록 (Multilingual Abstract)

Objectives : Deer antler Herbal-Acupuncture (DHA) solution represents one of the most commonly used medicine to treat rheumatoid arthritis. But, mechanisms of its antiarthritic activities are still poorly understood. Identification of common DHA aqua-acupuncture capable of affording protection or modulating the onset and severity of arthritis may have important human health implications.Results : We determined if DHA could prevent the binding of IL-1β to its cellular receptors. DHA addition to rat chondrocytes treated with IL-1β or with reactive oxygen species(ROS) prevents the activation of proteoglycan synthesis. After treatment with IL-1β, DHA increased the expression of mRNA encoding the type II IL-1β receptor. These results emphasize the potential role of two regulating proteins of the IL-1β signaling pathway that could account for the beneficial effect of DHA in osteRArthritis. The present study also identifies a novel mechanism of DHA-mediated anti-inflammatory activity. Conclusion : It is shown that DHA inhibits both IL-1β- and TNF-α-induced NO production in normal human articular chondrocytes. The observed suppression of IL-1-induced NO production is associated with inhibition of inducible NO synthase(iNOS) mRNA and protein expression. In addition, DHA also suppresses the production of IL-1-induced cyclooxygenase-2 and IL-6. The constitutively expressed cyclooxygenase-1, however, was not affected by the sugar. These results demonstrate that DHA expresses a unique range of activities and identifies a novel mechanism for the inhibition of inflammatory processes.

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