Enhancing antimicrobial activity of mastoparan-B by amino acid substitutions

다국어 초록 (Multilingual Abstract)

This study evaluated antimicrobial and hemolytic activities of mastoparan-B (MP-B) isolated fromthe venomof the hornet, Vespa basalis, and its analogs after substituting certain amino acid (aa) residues.MP-B exhibited significantly different antimicrobial activities against bacteria species/strains tested, especially two Escherichia coli strains, Staphylococcus xylosus and Citrobacter koseri at low dosages, and was non-specific against certain Gram-positive and -negative bacteria. Our results indicated that hydrophobicitymodification by single aa substitution may enhance their antimicrobial activities. An aa substituted MP-B, viz., MP-B-1, inwhich Trp substituted for Leu3, became more effective than the original peptide at inhibiting or killing the bacterial species tested, especially Klebsiella pneumonia, Salmonella typhimurium, and Salmonella Cholerasuis, and even up to 8 timesmore effective in some cases. However, MP-B-2 was virtually similar to MP-B against each bacterial species assayed,while MP-B-3 reduced its effectiveness greatly compared to others. On the other hand, MP-B and its analogs were not effective against the beneficial probiotics and they were not hemolytic to erythrocytes at the dosages tested. Our results suggested that MP-B becomes more potent against specific pathogenic bacteria and safe to the probiotics after undergoing appropriate amino acid substitutions.

참고문헌 (Reference)

1 Nakajima, T., "Wasp venom peptides; wasp kinins, new cytotrophic peptide families and their physico-chemical properties" 6 : 425-430, 1985

2 Mohammed Fayaz, A., "Vancomycin bound biogenic gold nanoparticles: a different perspective for development of anti VRSA agents" 46 : 636-641, 2011

3 Lu, Y., "Two families of antimicrobial peptides with multiple functions from skin of rufousspotted torrent frog" 27 : 3085-3091, 2006

4 Neil, E., "Towards the nonstick egg: designing fluorous proteins" 7 : 153-157, 2000

5 Brotz, H., "The lantibiotic mersacidin inhibits peptidoglycan synthesis by targeting lipid II" 42 : 154-160, 1998

6 Felgentreff, K., "The antimicrobial peptide cathelicidin interacts with airway mucus" 27 : 3100-3106, 2006

7 Kragol, G., "The antibacterial peptide pyrrhocoricin inhibits the ATPase actions of DnaK and prevents chaperone-assisted protein folding" 40 : 3016-3026, 2001

8 Patrzykat, A., "Sublethal concentrations of pleurocidin-derived antimicrobial peptides inhibit macromolecular synthesis in Escherichia coli" 46 : 605-614, 2002

9 Gottler, L. M., "Structure, membrane orientation, mechanism, and function of pexiganan - A highly potent antimicrobial peptide designed from magainin" 1788 : 1680-1686, 2009

10 Ho, C. L., "Structure and biological activities of a new mastoparan isolated from the venom of the hornet Vespa basalis" 274 : 453-456, 1991

11 Mendes, M. A., "Structural and biological characterization of three novel mastoparan peptides from the venom of the neotropical social wasp Protopolybia exigua(Saussure)" 45 : 101-106, 2005

12 Lin, C. H., "Structural and biological characterization of mastoparans in the venom of Vespa species in Taiwan" 32 : 2027-2036, 2011

13 Wu, T. M., "Stimulation of TNF-α, IL-1β and nitrite release from mouse cultured spleen cells and lavaged peritoneal cells by mastoparan M" 77 : 476-482, 1999

14 Russell, A. L., "Spectroscopic and thermodynamic evidence for antimicrobial peptide membrane selectivity" 163 : 488-497, 2010

15 Yu, K., "Relationship between the tertiary structures of mastoparan B and its analogs and their lytic activities studied by NMR spectroscopy" 55 : 51-62, 2000

16 Ostberg, N., "Protegrin structure–activity relationships : using homology models of synthetic sequences to determine structural characteristics important for activity" 26 : 197-206, 2005

17 Murayama, T., "Pertussis toxin-insensitive effects of mastoparan, a wasp venom peptide, in PC12 cells" 169 : 448-454, 1996

18 Murata, K., "Novel mastoparan and protonectin analogs isolated from a solitary wasp, Orancistrocerus drewseni drewseni" 37 : 389-394, 2009

19 Čeřovský, V., "New potent antimicrobial peptides from the venom of Polistinae wasps and their analogs" 29 : 992-1003, 2008

20 Hsueh, P. R., "New delhi metallo-β-lactamase-1 (NDM-1): an emerging threat among Enterobacteriaceae" 109 : 685-687, 2010

21 Yeaman, M. R., "Mechanisms of antimicrobial peptide action and resistance" 55 : 27-55, 2003

22 Park, C. B., "Mechanism of action of the antimicrobial peptide buforin II: buforin II kills microorganisms by penetrating the cell membrane and inhibiting cellular functions" 244 : 253-257, 1998

23 Yajima, Y., "Mastoparan-stimulated prolactin secretion in rat pituitary GH3 cells involves activation of Gq/11 proteins" 138 : 1949-1958, 1997

24 Gil, J., "Mastoparan, a novel mitogen for Swiss 3 T3 cells, stimulates pertussis toxin-sensitive arachidonic acid release without inositol phosphate accumulation" 113 : 943-950, 1991

25 Ohara-Imaizumi, M., "Mastoparan stimulates GABA release from MIN6 cells: relationship between SNARE proteins and mastoparan action" 289 : 1025-1030, 2001

26 Thennarasu, S., "Limiting an Antimicrobial Peptide to the Lipid − Water Interface Enhances Its Bacterial Membrane Selectivity : A Case Study of MSI-367" 49 : 10595-10605, 2010

27 Yang, X., "Isolation and characterization of a novel thermostable non-specific lipid transfer protein-like antimicrobial protein from motherwort (Leonurus japonicus Houtt) seeds" 27 : 3122-3128, 2006

28 Conceição, K., "Isolation and biochemical characterization of peptides presenting antimicrobial activity from the skin of Phyllomedusa hypochondrialis" 27 : 3092-3099, 2006

29 Andersen, B. M., "Is an increase of MRSA in Oslo, Norway, associated with changed infection control policy?" 55 : 531-538, 2007

30 Park, N. G., "Interaction of mastoparan-B from venom of a hornet in Taiwan with phospholipid bilayers and its antimicrobial activity" 36 : 793-801, 1995

31 Mahamat, A., "Impact of infection control interventions and antibiotic use on hospital MRSA: a multivariate interrupted time-series analysis" 30 : 169-176, 2007

32 Kavanagh, K., "Histatins: antimicrobial peptides with therapeutic potential" 56 : 285-289, 2004

33 Straub, S. G., "Glucose augmentation of mastoparan-stimulated insulin secretion in rat and human pancreatic islets" 47 : 1053-1057, 1998

34 Marsh, E. N. G., "Fluorine-a new element in the design of membrane-active peptides" 5 : 1143-1147, 2009

35 Yang, M. J., "Evaluating antioxidative activities of amino acid substitutions on mastoparan-B" 32 : 2037-2043, 2011

36 Lee, S. Y., "Effects of mastoparan B and its analogs on the phospholipase D activity in L1210 cells" 432 : 50-54, 1998

37 Andrews, J. M., "Determination of minimum inhibitory concentrations" 48 : 5-16, 2001

38 dos Santos Cabrera, M. P., "Conformation and lytic activity of eumenine mastoparan: a new antimicrobial peptide fromwasp venom" 64 : 95-103, 2004

39 Jeffery, G. S., "Computational protein design: engineering molecular diversity, nonnatural enzymes, nonbiological cofactor complexes, and membrane proteins" 15 : 452-457, 2011

40 Jeffery, G. S., "Computational protein design: Advances in the design and redesign of biomolecular nanostructures" 15 : 13-17, 2010

41 Hancock, R. E. W., "Clinical development of cationic antimicrobial peptides: from natural to novel antibiotics" 2 : 79-83, 2002

42 Jones, S., "Charge delocalisation and the design of novel mastoparan analogues: enhanced cytotoxicity and secretory efficacy of [Lys5, Lys8, Aib10]MP" 121 : 121-128, 2004

43 Mizuno, K., "Characterization of mastoparan-induced histamine release from RBL-2H3 cells" 36 : 447-456, 1998

44 Tavares, L. S., "Biotechnological potential of antimicrobial peptides from flowers" 29 : 1842-1851, 2008

45 Yoder, N. C., "Bioorthogonal noncovalent chemistry: fluorous phases in chemical biology" 10 : 576-583, 2006

46 Yonezawa, A., "Binding of tachyplesin I to DNA revealed by footprinting analysis : significant contribution of secondary structure to DNA binding and implication for biological action" 31 : 2998-3004, 1992

47 Ramamoorthy, A., "Beyond NMR spectra of antimicrobial peptides: Dynamical images at atomic resolution and functional insights" 35 : 201-207, 2009

48 Kamysz, W., "Are antimicrobial peptides an alternative for conventional antibiotics?" 8 : 78-85, 2005

49 Brogden, K. A., "Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?" 3 : 238-250, 2005

50 Isaacson, T., "Antimicrobial peptides with atypical structural features from the skin of the Japanese brown frog Rana japonica" 23 : 419-425, 2002

51 Lai, R., "Antimicrobial peptides fromskin secretions of Chinese red belly toad Bombina maxima" 23 : 427-435, 2002

52 Simmaco, M., "Antimicrobial peptides from amphibian skin: what do they tell us?" 47 : 435-450, 1998

53 Thennarasu, S., "Antimicrobial and Membrane Disrupting Activities of a Peptide Derived from the Human Cathelicidin Antimicrobial Peptide LL37" 98 : 248-257, 2010

54 Etzerodt, T., "Andresen, T.L., 2011. Selective acylation enhances membrane charge sensitivity of the antimicrobial peptide Mastoparan-X" 100 : 399-409, 2011

55 Zhang, W., "A novel analog of antimicrobial peptide Polybia-MPI, with thioamide bond substitution, exhibits increased therapeutic efficacy against cancer and diminished toxicity in mice" 31 : 1832-1838, 2010

56 Hirai, Y., "A new mast cell degranulating peptide "Mastoparan" in the venom of vespula lewisii" 27 : 1942-1944, 1979