Sperms, recovered from vaginal swabs, were processed to isolate high-molecular weight deoxyribonucleic acid(DNA) by the use of a buffer containing sarkosyl and DTT. They were digested with a particular restriction endonuclease, Pst 1. and analyzed wit...
Sperms, recovered from vaginal swabs, were processed to isolate high-molecular weight deoxyribonucleic acid(DNA) by the use of a buffer containing sarkosyl and DTT. They were digested with a particular restriction endonuclease, Pst 1. and analyzed with YNH and VI probes that recognize hypervariable regions within the human genomic DNA. The pattern of restriction fragment length polymorphisms(RELPs) with VNTR is identical to that observed with DNA prepared from white blood cells of male sexual partner. Therefore, DNA fingerprint with YNH and VI probe can be used to exclude or to determine the identity of an assailant in rape cases.