CC-NBS-LRR, a set of VvCRP markers, can distinguish cultivars with ripe rot resistance to Colletotrichum pathogens in grapevine

다국어 초록 (Multilingual Abstract)

Grape ripe rot is a fungal disease, which is almost inextinguishable particularly before and after harvest in most vineyards.
The management of this disease largely depends on the breeding of resistant varieties. Thus, the search for resistant varietiesshould parallel the molecular analysis of the markers associated with disease resistance. Here, we tested the leaf inoculationassay of Colletotrichum acutatum and C. gloeosporioides by inoculating them into 350 grapevine varieties. As a resultof the phenotypes common to both species, 119 varieties (34%) were classifi ed as resistant. Consequently, we discoveredthe locus with nucleotide-binding sites and carboxyterminal leucine-rich repeat regions ( NBS-LRR ) associated with graperipe rot resistance through genotyping-by-sequencing and genome-wide association study. Ultimately, we identifi ed theVitis vinifera Colletotrichum resistance protein ( VvCRP ) markers from chromosome 3, which belongs to the CC-NBS-LRRtype, as disease-resistant proteins. These markers were verifi ed as the melting peak, and their chromatograms of nucleotidesequences were visualized in each single nucleotide polymorphism (SNP) loci. All the SNP markers diff erentiated successfullythe resistant from the susceptible cultivars. Interestingly, we also found that a resistant cultivar named Bailey AlicanteA has a heterozygous genotype, substantiating its potential usefulness for crops such as grapevine with many hybrids. Altogether,our results indicate that VvCRP markers can be utilized to distinguish grape ripe rot-resistant cultivars, particularlyin crossbreeding populations.

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