冷藏血淸法과 韓國人 血淸脂質에 關한 硏究 = Study on the Chilled Serum Method and Blood Lipids in Koreans

다국어 초록 (Multilingual Abstract)

Classification of the hyperlipoproteinemias permits a rational approach to the diagnosis and management of the hyperlipidemic conditions which predispose to vascular diseases. However, the task of defining these disorders is not always simple and electrphoretic and ultracentrifugal apparatus are not available in most hospitals in Korea. Since the technique of chilled serum method is simple and may be feasible method in most hospitals, author modified the chilled serum method, which classifies the turbidity of chilled serum into 4 kinds of grade by visual assessment and investigated the relation between the grade of turidity of chilled serum and serum triglyceride level in 436 cases of normal and several diseases. In 241 of 436 cases classification of both hyperlipemias by the chilled serum method and hyperlipoproteinemias by agarose gel electrophoresis was carried out simultaneously and the results were compared with each other to ascertain the consistency between them.
The results obtained were as follows:
1. Serum triglyceride levels in (＋), (??), (??) and (??) turbidity grade by the semiquantitative chilled serum method were equivalent to 158.8±57.2(M±S.D.) ㎎％, 209.6±63.3㎎％, 260.8±65.2㎎％ and 408.5±103.7㎎％, respectively. The increment of mean serum triglyceride level between each turbidity was statistically significant. However, there was considerable overlapping among the normal, (＋) and (??) turbidity.
2. The lower limit of serum triglyceride level in (??) turbidity was equivalent to 231㎎％ and that in (??) turbidity was 302㎎％.
3. The incidences of hyperlipemias in normal Koreans by the chilled serum method was 19.6％ by the criteria of upper normal limits of 220㎎％ cholesterol and 140㎎％ triglyceride, and 9.0％ by the criteria of 255㎎％ cholesterol and 180㎎％ triglyceride. Endogenous hyperlipemia was frequently observed in angina pectoris, atherosclerotic heart disease, hypertension and diabetes mellitus, and hypercholeste-olemia in myocardial infarction. The exogenous and mixed hyperlipemias were not observed in both normal and several diseases.
4. The incidences of hyperlipoproteinemias in normal Koreans by the agarose gel electrophoresis were 14.0％ by the criteria of upper normal limits of 220㎎％ cholesterol and 140㎎％ triglyceride, and 8.0％ by the criteria of 255㎎％ cholesterol and 180㎎％ triglyceride. The type Ⅳ Hyperlipoproteinemia was frequently observed in angina pectoris, hypertension and diabetes mellitus, and type Ⅱa in myocardial infarction and cerebral hemorrhage. Type Ⅰ, Ⅱ and Ⅴ hyperlipoproteinemias were not observed in both normal and several diseases.
5. The majority of the hyperlipemias was consistent with the corresponding hyperlipoproteinemias.
6. Serum cholesterol and triglyceride levels in normal Koreans were 183.3±36.0(M±S.D.) ㎎％ and 116.1±32.4㎎％, respectively, in both sexes. Both serum cholesterol and triglyceride levels were higher significantly in angina pectoris, myocardial infarction, hypertension and diabetes mellitus than in normal. Serum cholesterol was increased in cerebral hemorrhage and triglyceride in atherosclerotic heart disease.
7. The ratios of lipoprotein fraction in normal Koreans were alpha-lipoprotein: 35.8±5.6％, beta-lipoprotein: 42.5±6.1％, and prebeta-lipoprotein: 21.0±5.2％.
The prebeta-lipoprotein fraction was significantly increased in angina pectoris and hypertension, and beta-lipoprotein in myocardial infarction. The alpha-lipoprotein fraction was significantly decreased in angina pectoris, myocardial infarction, atherosclerotic heart disease, valvular heart disease, hypertension and diabetes mellitus.

목차 (Table of Contents)

• Ⅰ. 緖論
• Ⅱ. 硏究對象 및 方法
• Ⅲ. 硏究成績
• 1. 冷藏血淸의 白濁度와 血淸 triglyceride 및 cholesterol 値
• 2. 冷藏血淸法에 依한 高脂血症의 正常人 및 各種 疾患에서의 頻度
• 3. Agarose gel 電氣泳動法에 衣한 高脂蛋白血症의 正常人 및 各種 疾患에서의 頻度
• 4. 冷藏血淸法에 依한 高脂血症과 agarose gel 電氣泳動法에 依한 高脂蛋白血症의 比較
• 5. 正常人의 血淸 triglyceride 및 cholesterol 値와 lipoprotein 分劃 및 그 比
• 6. 各種 疾患에서 血淸 triglyceride 및 cholesterol 値와 lipoprotein 分劃 및 그 比
• Ⅳ. 考按
• Ⅴ. 結論
• 參考文獻