Kanamycins, pseudotrisaccharides antibiotics, have three closely related structural form namely kanamycin A, kanamycin B and kanamycin C in which 2-DOS is substituted at the C-4 position by an aminoglucose functionality: 6-amino-6- deoxy-D-glucose, ne...
Kanamycins, pseudotrisaccharides antibiotics, have three closely related structural form namely kanamycin A, kanamycin B and kanamycin C in which 2-DOS is substituted at the C-4 position by an aminoglucose functionality: 6-amino-6- deoxy-D-glucose, neosamine, and D -glucosamine respectively and at the C-6 position by 3-amino-3-deoxy-D-glucose (kanosamine). Here, we have Engineered Streptomyces lividans TK24 by expressing two sets of rebombinant plasmids pSK-7 and pSK-17 responsible for the production of paromamine and kanamycin C respectively. Bio-active secondary metabolite from pSK-17/SL against Bacillus subtilis and ESI/MS,LC/MS and ESI/MS/MS at 485 [M+H]+ were the evidences for the production of Kanamycin C.