Microbacterium rhizosphaerae sp. nov., isolated from a Ginseng field, South Korea

다국어 초록 (Multilingual Abstract)

<P>A novel Gram-stain positive, aerobic, short rod-shaped, non-motile bacterium, designated strain CHO1(T), was isolated from rhizosphere soil from a ginseng agriculture field. Strain CHO1(T) was observed to form yellow colonies on R2A agar medium. The cell wall peptidoglycan was found to contain alanine, glycine, glutamic acid, d-ornithine and serine. The cell wall sugars were identified as galactose, mannose, rhamnose and ribose. Strain CHO1(T) was found to contain MK-11, MK-12, MK-13 as the predominant menaquinones and anteiso-C-15:0, iso-C-16:0, and anteiso-C-17:0 as the major fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, an unidentified phospholipid and three unidentified glycolipids were found to be present in strain CHO1(T). Based on 16S rRNA gene sequence analysis, strain CHO1(T) was found to be closely related to Microbacterium mangrovi DSM 28240(T) (97.81 % similarity), Microbacterium immunditiarum JCM 14034(T) (97.45 %), Microbacterium oryzae JCM 16837(T) (97.33 %) and Microbacterium ulmi KCTC 19363(T) (97.10 %) and to other species of the genus Microbacterium. The DNA G+C content of CHO1(T) was determined to be 70.1 mol %. The DNA-DNA hybridization values of CHO1(T) with M. mangrovi DSM 28240(T), M. immunditiarum JCM 14034(T), M. oryzae JCM 16837(T) and M. ulmi KCTC 19363(T) were 46.7 +/- 2, 32.4 +/- 2, 32.0 +/- 2 and 29.2 +/- 2 %, respectively. On the basis of genotypic, phenotypic and phylogenetic properties, it is concluded that strain CHO1(T) represents a novel species within the genus Microbacterium, for which the name Microbacterium rhizosphaerae sp. nov. is proposed. The type strain of M. rhizosphaerae is CHO1(T) (= KEMB 7306-513(T) = JCM 31396(T)).</P>