유전적으로 변이된 흰쥐 섬유아세포에 의한 L-dopa 및 도파민의 실험관내 분비 = In Vitro Release of L-dopa and Dopamine by Genetically Modified Fibroblasts of the Rat

다국어 초록 (Multilingual Abstract)

A construct DNA containing the cDNA for rat tyrosine hydroxylase(TH) and another construct DNA containing the cDNA for bovine L-dopa decarboxylase(DDC) were made and those genes were transferred-,by calcium phosphate transfedon method into the immortalized rat fibroblasts. A both TH- & DDC- positive clone was identified by immunocytochemical staining. These cells produced L-dopa and dopamine and released them into the cell culture medium in vim. which was confirmed by high performance liquid chromatography. These results have general implications for the application of gene therapy and specific implications for Parkinson disease.