Effect of Collagen Scaffold with Adipose-derived Stromal Vascular Fraction Cells on Diabetic Wound Healing: A Study in a Diabetic Porcine Model

다국어 초록 (Multilingual Abstract)

Diabetes can impair neovascularization important for wound healing, and this study investigated the hypothesis that this effect can be reversed by an active provisional matrix. Here, a 3D collagen scaffold and uncultured adipose-derived SVFs were firstly utilized to construct a collagen-targeting system for wound healing in a diabetic porcine model. The diabetic porcine models were made by injected streptozocin (STZ) intravenously and removed full thickness skin from the dorsum. Each animal received four different samples: Group A (control groups, n=24), Group B (SVFs groups, n=24), Group C (scaffold groups, n=24), Group D (SVFs -scaffold groups, n=24). PBS was applied to the wounds in Group A. 1×105 SVFs suspended in PBS were sprayed on the wounds in Group B. Scaffolds only with PBS were covered on the wounds in Group C. Scaffolds with equal volume of cell suspension containing 1×105 SVFs were covered on the wounds in Group D. The healing rates were calculated and compared among the groups and the tissues of the wound were taken and evaluated for histological analysis. The diabetic wound treated with the SVFs -collagen scaffold showed a significant decrease in wound size, an increase of neovascularization and a maximum VEGF and bFGF expression in comparison with SVFs and scaffold alone. The SVFs-collagen scaffold accelerates wound healing since it stimulates higher capillary formation causes major proangiogenesis factors expression in diabetic pigs suffering from skin defects, suggesting this approach could have utility for diabetic wound healing.

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